烟霉

Objective To establish an ELISA for detecting specific antibody against Aspergillus fumigatus, and to evaluate its usefulness in diagnosis of invasive aspergillosis.

目的 建立检测人血清中抗烟曲霉特异性抗体的ELISA法，评估该法在侵袭性烟曲霉病诊断中的价值。

Objective To investigate the effects of cinnamaldehyde and citral on DNA and RNA of Aspergillus flavus and A. fumigatus cells and their mechanisms. Methods A.

目的 研究肉桂醛、柠檬醛对黄曲霉和烟曲霉细胞DNA、RNA水平的影响，以期探明其抗真菌的作用机制。

Methods BALB/c mice were immunized by cytoderm antigen of Aspergillus fumigatus, excreting antigen and inactivated conidiospore respectively, to prepare monoclonal antibodies specific to Aspergillus. Cross-reactions between mAbs and antigens from Aspergillus or Monilia genus were characterized by immunofluorescence.

采用烟曲霉细胞壁抗原、分泌抗原和灭活分生孢子，分别免疫BALB/c小鼠，制备特异性的抗曲霉单克隆抗体，免疫荧光法鉴定单克隆抗体与曲霉属和念珠属抗原的交叉反应。

Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果：糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强，菌落的自发荧光在1小时内逐渐减弱；3％戊二醛溶液、pH4.0盐酸溶液、1280μg／mL和64μg／mL氟康唑溶液、3％美蓝溶液处理后马拉色菌自发荧光都增强，10％氢氧化钾溶液中的马拉色菌在开始时荧光增强，2天后减弱；白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光，但都较马拉色菌弱。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌，接种在Dixon培养基32℃培养，分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光；分别用3％戊二醛溶液、pH4.0盐酸溶液、10％氢氧化钾溶液、1280μg／mL和64μg／mL氟康唑溶液、0.3％美蓝溶液处理后观察马拉色菌自发荧光的变化；选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

Mold of 34 species were isolated from moldy books.Among them,3 species which were demonstrated to be the key mould causing book mold were identified morphologically and molecularly as Chaetomium globosum,Aspergillus terreus and Aspergillus fumigatus.

从霉变的书籍中分离纯化了34种霉菌，对其中的3种主要霉菌进行了形态学和分子生物学鉴定，分别鉴定为球毛壳菌种、土曲霉种、烟曲霉种。

Results The dermatophagoides farinae and pteronyssinus (62/118) were the most common allergen in inhalation group in 118 patients with allergic disease. Dogly or feline scurf was the next (35/118), then were ambrosia artemisiiofolia and muguort (14/118), cockroach(12/118), cypress, elm, Surname. Cottenuood (8/118), humulus scandens (7/118), polyvalent fungus(6/118). In food group the most common allergen was mutton or beef (13/118), then was cashew, peanut, soybean (11/118), wheat (10/118),milk (9/118), fish, shrimp, crab(5/118), mango(4/118),egg, chicken., yolk(1/118).The positive rates of total IgE level was 35.59%.

结果 118例变应性鼻炎患者中吸入组过敏原以以屋尘螨粉、尘螨(62/118)为最高，其后依次为猫毛皮屑、狗毛皮屑(35/118)、矮豚草、蒿(14/118)、蟑螂(12/118)、柏榆、梧桐、柳三角叶杨(8/118)、律草(7/118)、点青霉、分枝孢霉、烟曲霉、交链孢霉(6/118)；食入组过敏原以牛肉、羊肉(13/118)为最高，其后依次为腰果、花生、黄豆(11/118)、小麦(10/118)、牛奶(9/118)、鱼虾蟹(5/118)、芒果(4/118)、鸡蛋白、鸡蛋黄(1/118)；总IgE阳性率为35.59%。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。