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The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体,并通过农杆菌将上述基因转入烟草和拟南芥中,得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验,结果表明,双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生,再生芽数量平均可达13~16个/cm双鳞片,比传统的鳞茎盘之间分化提高30~50%;鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织,但以带花药的花丝诱导出的愈伤组织最好。

In this study, what had been used inculding CBF1 and CBF3 of CBF family, its downstream COR15a gene , regulating element CRT/DRE in promoter of COR15a gene, Cloning CBF1 and CBF3 from Arabidopsis thaliana, and Cloning ApCOR15a gene from Arabis pumila of xinjiang. On the basis of plasmid pBI121,being used CaMV35S promoter and SAR iductivity promoter synthesized by PCR, six plant expression vectors had been constructed: pCB111, pCB111-1, pCB112,pCB112-1,pCB113, pCB113-1, and then transformed to Agrobacterium GV3101 by electics.

本研究选用了CaMV35S组成型启动子和人工合成的由水杨酸诱导的启动子SAR、拟南芥CBFs家族中的CBF1和CBF3、新疆小拟南芥COR15a基因及其CRT/DRE顺式作用元件构建了不同组合的六个植物表达载体:pCB111、pCB111-1、pCB112、pCB112-1、pCB113和pCB113-1,并采用农杆菌介导的叶盘转化法转化烟草,获得了大量的抗性植株。

Malignancy following enteric bladder augmentation arose in 4.5%(7/153) of our patients and was associated with coexisting carcinogenic stimuli (prolonged tobacco/chronic immunosuppressive exposure), or alternatively with the inherent risk of malignancy existing with bladder exstrophy.

我们研究的接受肠道膀胱扩大术的患者有4.5 %( 7 / 153 )发生了恶性肿瘤,恶性肿瘤的发生与共存的致癌刺激(长期烟草/慢性免疫抑制剂的服用)有关,或者与膀胱外翻固有的恶性肿瘤发生风险相关。

To the new and old customers better service, I Wafangdian Bearing Group Company and / Luoyang Bearing Group and the Luoyang Bearing Institute, famous and all major manufacturers have established good cooperative relations between suppliers, our company can meet new and old customers High-precision bearings on the demand for imports bearing sales NACHI, SKF, NSK, NTN, IJK, IKO, Japan, spherical bearing FYH, Germany SWC high temperature bearings, the British tobacco RHP high-precision machinery bearings, Germany FAG28000 / hours of high-speed, High-precision bearings, chemical equipment for the U.S. Fafnir Bearing, Japan EZO corrosion-resistant stainless steel bearings; NORGREN, SIEMENS imports of machinery and other accessories, the United States JOHN CYANE mechanical seals, Germany KUKKO bearing working tools, triangular belt, Q, H series Shotblast cleaning machine , And other products.

为了对新老客户更完善的服务,我公司与瓦房店轴承集团/洛阳轴承集团和洛阳轴承研究所,及各大名优厂家建立了良好的供货合作关系,我公司还可满足新老客户对高精密轴承的需求,进口轴承销售NACHI、SKF、NSK、NTN、IJK、IKO、日本外球面FYH轴承、德国耐高温SWC轴承、英国RHP高精度烟草机械轴承、德国FAG28000/分的高转速、高精度的轴承、美国Fafnir化工设备专用轴承,日本EZO耐腐蚀不锈钢轴承;NORGREN、 SIEMENS等进口机械配件,美国JOHN CYANE机械密封、德国KUKKO轴承装卸工具、三角皮带、Q,H系列抛丸清理机等产品。

A dark, aromatic, small-leaved tobacco of eastern Europe and used in cigarettes.

用于香烟的黑的、芳香的、小叶的、东欧烟草。

It was demonstrated that the deduced GhMADS1 protein was highly homologous to the AGL2 group of MADS-box proteins from Vitis vinifera, Nicotiana sylvestris, Petunia hybrida, Arabidopsis thaliana and Anntirhinum majus.

该片段(GhMADS1)长713 bp,包含一个711 bp的开放阅读框,推导的氨基酸序列(236个氨基酸)与葡萄、烟草、矮牵牛、拟南芥和金鱼草等的AGL2组MADS框蛋白有很高的序列相似性。

However restaurateurs and bar operators say the rules will be hard to implement because of the high costs involved.

报道说,有多达70%的印尼男子均爱吸一种名为&kretek&的味道刺激的丁香卷烟,同时该国也是全世界利润最为丰厚的烟草市场之一。

Will toughen sanctions for selling to minors; Amanda Sandford of ASH, an anti-smoking lobby group, would like to see sales restricted to licensed tobacconists, as in much of Europe.

议会正准备通过一项法案,准备更加严厉的制裁卖烟给未成年人的商家。,来自反对吸烟游说组织-'烟灰'的阿曼达。斯坦福希望烟草销售只限于有执照的烟草商,就象大部分欧洲国家那样。

In Malawi alone there are an estimated 78,000 boys and girls employed in tobacco harvesting. On average they earn 17 cents for a 12-hour day of back-breaking, bare-handed work, according to a recent report from Plan International.

有人认为全世界有千千万万的孩子在烟草场做全职,他们暴露在大量的有毒的尼古丁下,在备受辱骂的劳动环境中遭受苦难。

Tobacco of China,3:44-47[13]Alvarez M.E., Penell R.I.,Meijer P.J., Ishikawa A., Dixon R.A. and Lamb C.1998.Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity.Cell,92:773-784

已知H2O2是活性氧的典型代表,因此,我们推测,AT-毒素诱导的烟草对TMV的抗性,可能是由于毒素诱导的HCD激活了SAR通路,从而实现了抗病性,详细机制还在进一步研究。

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你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。