烟草的

Tobacco alimentation and fertilizing research are developed very fast during those 10 years in china.

近10年中国烟草营养与施肥研究有较快发展，施肥以优质、高产、高效、生态、安全为目标，全面进入生产与生态并重的施肥阶段。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

In this section of our website, therefore, you will find information on these smokeless tobacco-related messages, as well as links to government and public health authorities, including the U.S. Surgeon General's reports related to tobacco.

在这一节我们的网站，因此，你会发现，这些资料无烟烟草有关的讯息，以及链接的政府部门及公共卫生当局，包括美国在内的外科医生秘书长的报告有关的烟草。

The change in net income was primarily attributable to a $26.9 million decrease in research and development expenses related to the cessation of the development of Sulonex in March 2008, a $5.4 million decrease in research and development expenses related to KRX-0401, a $20.8 million increase in license revenue related to an amendment to the September 2007 sublicense agreement with Japan Tobacco Inc. and Torii Pharmaceutical Co., Ltd. which eliminated the Company's significant ongoing obligations included in the original agreement ($18.0 million) and a $3.0 million milestone payment from JT/Torii earned in the first quarter of 2009, and a $3.6 million increase in other revenue primarily related to the settlement of a dispute for $3.5 million.

在净收益的变化主要是由于2008年3月，在研究和开发费用与韩国证券期货交易所，0401（perifosine五百四十〇点〇〇万美元减少到1的研究和有关的发展的Sulonex停止开发费用二千六百九十点〇〇〇万美元减少），在涉及对2007年9月与日本烟草公司和鸟药业有限公司转授权协议，消除了公司的重大，持续的义务的修正案，授权收入二千零八十零点零零零万美元增加原协议中（$ 18.0万美元），由日本烟草300万美元的里程碑付款/鸟赚了2009年第一季度，并在其他收入主要是与一个以350万美元的争端解决三六○○○○○美元增加。

It can improve the conflagrant properies of tobacco when cobalt joined, and the min-nim selenium can reduce the deleterious substance in the cigcarette haze, and reduce the harm to health,therefore in this paper we have researched the adsorptive properities of cobalt on tobacco and some factors which affect the adsorption of selenium on tobacco by the theory of liquid - solid adsorption . We have discussed the influences of the adsorbate concentration. temperature, pH and certain concomitant substance, and have determined adsorption isothermal under the different temperature.

微量元素钴的加入能改善烟丝的燃烧性能；微量硒的存在能降低烟气中有害物质，减轻吸烟对人体健康的危害，因此本论文利用固—液吸附理论研究了钴在烟草上的吸附性能以及硒在烟草上吸附的一些影响因素，探讨了吸附质浓度、温度、pH及某些共存物质对吸附的影响，测定了不同温度下的吸附等温线。

The fungicidal activity of methanol extract was higher than that of ethyl acetate extract, and the inhibition rates were higher than 90% against Alternaria longipes, Fusarium oxysporium, Gibberella zeae, Glomerella cingulata, Exserohilum turcicum under the concentration of 1000g/mL The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, Exserohilum turcicum and Fusarium coeruleum, the EC50 values was 59.4g/mL, 96.5g/mL and 132.5g/mL. In the bioassay of the fermentation products against Bacillus sub tills and Bacillus cereus, by means of detection fo inhibition zone, the inhibition zone diameter was 20mm and 27mm. The results of pot tests that methanol extract showed higher than 70% of protective efficacy and therapeutic efficacy against wheat powdery mildew under the concentration of 2000g/mL.

其中，甲醇提取物的抑菌活性比乙酸乙酯提取物强，在1000g/mL浓度下对烟草赤星病菌、马铃薯干腐病菌、小麦赤霉病菌、苹果炭疽病菌、玉米大斑病菌的菌丝生长抑制率均大于90%；抑制孢子萌发法试验结果表明，2K3菌株菌丝体的甲醇提取物对烟草赤星病菌玉米大斑病菌和马铃薯干腐病菌的EC50分别为59.4g/mL、96.5g/mL和132.5g/mL；管碟法生测结果表明，2K3菌株菌丝体的甲醇提取物对枯草芽孢杆菌和蜡状芽孢杆菌的抑菌圈直径分别为20mm和27mm；盆栽试验结果表明，2K3菌株菌丝体的甲醇提取物在2000g/mL浓度下对小麦白粉病的治疗和保护作用均在70%以上。

In preferred embodiments, the resultant tobacco products are dried, golden-yellow leaves having almost negligible amounts of the known carcinogens NNN and NNK, in comparison to conventionally cured tobacco.

在优选的实施方案中，所得的烟草制品为金黄色的干烟叶，与常规熟化的烟草相比其已知的致癌物NNN和NNK的量几乎可忽略不计。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

The preliminary bioassay indicated that the pyridine compounds display inhibitory activities against mosquites; pyrazolopyridine compounds possessed some plant growth regulation activity; pyrazole compounds displayed some inhibitory activities against wheat rust and tobacco mosaic virus respectively.

初步的生物活性测定结果表明，吡啶类化合物对蚊子有较好的杀虫作用；吡唑并吡啶类化合物有一定的植物生长调节活性；部分吡唑类化合物对小麦锈病和烟草花叶病毒有较好的抑制效果；部分1，2，3-噻二唑类化合物对烟草花叶病毒有很好的抑制效果，与商品药剂病毒A的效果相近。

This light, soft sweet cake is designed to appeal to many smokers of Matured Virginias who have been unable to endure aromatics but who would prefer a tobacco that smells sweeter in the room, as well as smokers of traditional aromatic cavendishes that would like a cooler dryer smoking tobacco.

这个浅色，柔软的甜蛋糕是有计划的去满足那些成熟维吉尼亚烟客，他们不能容忍有芳香味的，宁愿选择那些在房间闻起来有甜味的烟草，那些喜欢传统芳香的卡文迪西的也许会喜欢寒冷干燥的烟草。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。