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The optimum conditions for extraction was proved practicable by condition filtration and orthogonal design with supersonic wave method .The results showed that the optimum conditions for extraction to ultrasonic method is : methanol (φ=40,20ml),and 15℃ for 30 min ,this paper has determined nicotine contents that is 23.50mg/g at absorption peak (λ=262nm) by HPLC with an eluent consisting of methanol –potass Phosphate alleciate (pH=3) with the recovery and coefficient of variation are 101.52% and 0.91%.

通过超声波提取方法进行条件筛选和正交化实验,确定了烟草中尼古丁的最佳提取条件,结果表明,超声波最佳条件是:甲醇浓度40%,固液比1:20,提取时间30 分钟,超声温度15℃,用高效液相色谱方法在波长262nm 处测定尼古丁的含量,流动相组成为甲醇:磷酸二氢钾缓冲溶液=10:90,测得烟草中尼古丁的含量为23.50 mg/g,平均回收率为101.52%,相对偏差为0.91%。

During processing tobacco, baking cut tobacco step directly influenced tobacco quality, and temperature control was the key of the step.

在烟草加工过程中,烘丝环节直接影响烟草质量的好坏,而对温度的控制是烘丝环节的关键所在。

Undiversified tobacco companies stand to lose if there were a mass migration to smokeless products like Snus.

如果有大量的如鼻烟这样的无烟烟草产品流入,一成不变的烟草公司将亏损。

PCR and Southern blot analysis confirmed stable integration of the foreign genes into the chloroplast genomes of T_0 and T_1 transgenic plants, and homoplasmy of T_1 transgenic plants.

只转化phaC2基因的叶绿体型转基因烟草基本检测不到有mel-PHAs合成,共表达phaC和phaG基因的叶绿体型转基因烟草则能合成相对较高水平的mcl-PHAs。

A full-length cDNA encoding sucrose: sucrose 1-fructosyltransferase from Lactuca sativa was inserted into pCAMBIA1300-als under the control of the CaMV 35S promoter. This plasmid was used for Agrobacteriummediated tobacco leaf disc transformation. Transgenic plants were analyzed by PCR and Southern blotting. 1-SST gene expression was confirmed by RT-PCR.

将从莴苣中克隆的1-SST基因重组到pCAMBIA1300-als中,构建了在CaMV 35S启动子调控下的植物表达载体,利用农杆菌介导的叶盘转化法将1-SST基因导入烟草中,PCR和Southern杂交检测表明获得了转基因植株,RT-PCR结果表明该基因在烟草中正常表达。

Tobaccos are consumption goods which can satisfy peoples physiological and psychological needs through the intake of smoke from burning.

烟草制品是一类特殊的消费品,吸烟者主要是通过吸食烟草燃烧产生的烟气来获得生理和心理上的满足。

In the process of establishing the Yunnan tobacco material deal on net system, above-said relative information technology and standard has been used, and draw the architecture of the system and the solution of some key problem in the system.

结合云南省烟草物资供应系统项目的实施,本论文将上述相关技术、标准应用其中,给出了基于XML和中间件技术的烟草物资交易系统的体系结构设计,并对项目实施中关键问题提出了具体解决方案。

Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

一些有香味很棒的一年生植物有香雪球、大型开花烟草&普通烟草&、招展丛生的喇叭形白色花朵帝王百合以及柔软的紫色向日葵。

Scented plants can also evoke memories. Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

芳香的植物同样能唤起回忆,一些一年生植物有很好的香味,比如香雪球,大片花叶烟草植物:美花烟草,花攒锦簇,随风摇曳的白色&帝王百合&,和像天鹅绒般的紫色&青莲&。

Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

香雪球,大花烟草&美花烟草&,喇叭状白色花招摇的帝王百合,以及紫色的天鹅绒向日花是香气逼人的一年生植物。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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