烟草的

A method for chiral analysis of anatabine,nornicotine and anabasine in tobacco has been established.

建立了烟草中新烟草碱、降烟碱和假木贼碱的手性分析方法。

Standard curve and standard addition methods were applied for the quantitative analysis of potassium in tobacco. The main advantage of this method is that the tobacco powder can be directly injected as suspended sample, and has no need for cineration in advance.

该方法最突出的优点是烟草样品不需灰化处理，烟草样品粉末与悬浮剂作用直接进样测试，节约了前处理时间和试剂。

Tobacco mosaic virus RNA and Cucumber mosaic virus were extracted and sequenced, respectively.

分别提取烟草普通花叶病和烟草黄瓜花叶病的病毒RNA。

Japan Tobacco, which purchased Gallaher Group Plc for 7.5 billion pounds ($11.4 billion) in 2007, will not make another similar-sized acquisition in the next three years, Shimizu said.

日本烟草公司在2007年以75亿英镑（合114亿美元）收购了加莱赫集团，Shimizu说，在未来三年内，日本烟草不会再进行类似规模的收购活动。

Trivedi outlined various initiatives of the Government of India to control tobacco consumption which kills about 9 lakh people every year in the country.

Trivedi概述了印度政府控制烟草消费的各种措施，在印度烟草每年导致90万人。

Countrywide National People's Congress also passes sanction " convention of nicotian control frame ", mean domestic nicotian industry to will bear more comes from convention manacle.

全国人大也通过批准了《烟草控制框架公约》，意味着国内烟草行业将承受更多来自公约的束缚。

Report from our correspondent the reporter learns from city nicotian firm yesterday, this month 13 days, close half an year of course of substation of city tobacco high new developed area is visited difficult and meticulously, feel a platoon, dog, expect, in this city 39 successes destroy clear Jiang Donglu one is registered through cleaning out treasure net, the name is " excessive popular cause ", advocate the battalion smoke, false smoke of wine sells nest site.

本报讯 记者昨日从市烟草公司获悉，本月13日，市烟草高新区分局经过近半年艰苦细致地走访、摸排、跟踪、守候，在本市清江东路39号成功摧毁一个通过淘宝网注册，名为"溢香源"，主营烟、酒的假烟销售窝点。

A retail dealer in tobacco and tobacco related articles.

做烟草和与烟草有关生意的零售商。

Our previously study has been showed that transgenic N.benthamiana and N.tabacum plants expressing TYLCCNV DNAβC1 developed pleiotropic morphologic abnormalities such as leaf curling,distortion and interveinal protuberances on the abaxial surface. To determine whether theβC1 protein of TbCSY is also responsible for symptom induction,we prepared transgenic N.benthamiana and tomato plants expressing the TbCSV DNAββC1.Of the rooted plantlets,only 10%of transgenic N.benthamiana plants were moderately abnormal,including upward leaf curling.

DNAβ互补链上编码一个保守的ORF，即βC1，对TYLCCNV DNAβ的βC1的突变发现它编码一个症状决定因子，表达TYLCCNV DNAβ的βC1的转基因烟草产生曲叶、畸形和叶背面组织增生等发育异常的表型，而大部分转化TbCSV DNAβ的βC1基因的烟草和番茄植株均与正常植株类似，只有少数烟草表现出轻微叶上卷表型。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术，研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异，初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带：晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合，分别为SF25*SR2和SF3*SR26；晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14；NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14，烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合，研究了普通烟草不同类型间的亲缘关系，将普通烟草类型间8个不同供试材料划分为3类别。I：烤烟类型G-28II：白肋烟类型(MD40、By21、大白筋599III：香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。