烟草

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

In this study, what had been used inculding CBF1 and CBF3 of CBF family, its downstream COR15a gene , regulating element CRT/DRE in promoter of COR15a gene, Cloning CBF1 and CBF3 from Arabidopsis thaliana, and Cloning ApCOR15a gene from Arabis pumila of xinjiang. On the basis of plasmid pBI121,being used CaMV35S promoter and SAR iductivity promoter synthesized by PCR, six plant expression vectors had been constructed: pCB111, pCB111-1, pCB112,pCB112-1,pCB113, pCB113-1, and then transformed to Agrobacterium GV3101 by electics.

本研究选用了CaMV35S组成型启动子和人工合成的由水杨酸诱导的启动子SAR、拟南芥CBFs家族中的CBF1和CBF3、新疆小拟南芥COR15a基因及其CRT/DRE顺式作用元件构建了不同组合的六个植物表达载体：pCB111、pCB111-1、pCB112、pCB112-1、pCB113和pCB113-1，并采用农杆菌介导的叶盘转化法转化烟草，获得了大量的抗性植株。

Just like alcohol, tobacco, coffee and sakau, Areca nut and Betel leaf are culturally-approved psychoactive drugs.

就像酒精，烟草，咖啡和sakau，槟榔和槟榔叶在文化上认可的精神科药物。

Also known as Leopards Bane, Wolf's Bane, and Mountain Tobacco, Arnica is also available in tincture, pill and injectable forms for internal use.

另外，作为巴豹，狼的危害，烟草和山，阿尼卡知道的是还可以在酊，丸和供内部使用注射方式。

The research of aromatic matter of tobacco was paid more attention in recent years.

烟草香气物质在近年来的研究中一直倍受重视。

Follow the prompts to arrive at a final TMV price with options for the exact car you are buying.

按照提示达成最后烟草花叶病毒与确切的车，你买期权的价格。

Our products are widely used in metallurgy, mining, building materials, foundry, chemicals, tobacco, asphalt, cement, machinery, food, machinery processing, boiler and other industries, and to achieve the design - manufacturing - Installation - Debugging - the through-train service technical training .

本公司产品广泛应用于冶金、矿山、建材、铸造、化工、烟草、沥青、水泥、机械、粮食、机械加工、锅炉等行业，并且实现了设计—制造—安装—调试—技术培训的一条龙服务。

The company's products are widely used in metallurgy, mining, building materials, foundry, chemicals, tobacco, asphalt, cement, machinery, food, machining, boiler and other industries, and achieve the design - Manufacturing - Installation - Commissioning - Technical training of one-stop Service .

本公司产品广泛应用于冶金、矿山、建材、铸造、化工、烟草、沥青、水泥、机械、粮食、机械加工、锅炉等行业，并且实现了设计-制造-安装-调试-技术培训的一条龙服务。

In this study, BADH, gene encoding the key enzyme in betaine biosynthesis, which was isolated from Atriplex hortensis, and SeNHX1, a vacuole Na+/H+ anitiporter in Salicornia europhaea L., were used to transform the tobacco to improve the salt tolerance.

本研究拟用耐盐性较强植物山菠菜中的甜菜碱合成关键基因BADH和盐生植物盐角草的液泡膜Na+/H+ anitiporter基因SeNHX1对模式植物烟草进行转化，以确定其各自在耐盐性方面所起的作用。

Genes encoding Drought-Responsive-Element Binding proteins were cloned from Triticum aestivum and Atriplex hortensis. TaDREB1 and AhDREB1 can specifically bind the DRE elements in the promoters and thus activate the transcription of downstream genes in yeast cells as well as transgenic Arabidopsis and transgenic tobacco plants.

克隆了小麦和山菠菜转录因子TaDREB1和AhDREB1基因，它们都编码DRE（Dehydration-Responsive-Element）结合蛋白，在酵母单杂交系统、转基因拟南芥和转基因烟草中都能特异结合DRE元件并激活下游报告基因的表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。