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The Klason lignin level of anti-4CL-transformed tobacco was much lower than that of the control. The cellulose content increased in transgenic tobacco with UGPA constructs.We also obtain transgenic tobacco transformed with Acetobacter xylinum UGPase gene.

研究了木醋酸菌来源的UGPase基因与植物来源的UGPA基因在转基因烟草中的表达,测定了两种转基因烟草的尿苷二磷酸葡萄糖焦磷酸化酶的活性、木质素含量和纤维素含量。

On the antagonistical mechanism,it is suggested that the poison of Cd on tobacco could be impaired by Fe supply in Cd pollution area.

结果表明:Cd、Fe、Cd+Fe和Fe+Cd处理均不改变氨基酸的种类组成;Cd对烟草叶片氨基酸含量的影响比Fe大;Fe抑制Cd对氨基酸含量的影响,Cd也抑制Fe的作用,Cd与Fe相互拮抗,并初步探讨了拮抗机制;建议在Cd污染地区通过施加Fe来减轻Cd对烟草的毒害。

The results showed that aphidicide had low effect on tobacco virus spread by aphids, virusicide effect were about 60%;the occurrence degree and index of disease were lower when the transplanting time was put off.

结果表明,杀蚜剂防治烟草蚜传病毒病效果较差,病毒抑制剂效果一般为60%左右;随移栽期推迟,烟草蚜传病毒病发病显著减轻。

The tobacco were sprayed by virusicide, aphidicide in the field and using different transplanting time in order to control the tobacco virus spread by aphids.

烟草炼苗期采用杀蚜剂、病毒抑制剂、纱网罩烟苗,田间生长期采用杀蚜剂、病毒抑制剂和不同移栽期防治烟草蚜传病毒病。

The large stem and loop structure can be constructed from the sequence surrounding the 5' and 3' ends of the 16S rRNA gene.The stem is about h alf shorter than that of tobacco,due to a 79 bp deletion in Brassica napus chlor oplast.

5%、96.1%。油菜叶绿体16SrRNA基因5'端上游及3'端下游的顺序能互补而形成一个较大的茎环结构,但与烟草相比,由于3'端下游顺序有79bp的缺失,因此,该结构中的茎部分大小仅为烟草的二分之一。

It can improve the conflagrant properies of tobacco when cobalt joined, and the min-nim selenium can reduce the deleterious substance in the cigcarette haze, and reduce the harm to health,therefore in this paper we have researched the adsorptive properities of cobalt on tobacco and some factors which affect the adsorption of selenium on tobacco by the theory of liquid - solid adsorption . We have discussed the influences of the adsorbate concentration. temperature, pH and certain concomitant substance, and have determined adsorption isothermal under the different temperature.

微量元素钴的加入能改善烟丝的燃烧性能;微量硒的存在能降低烟气中有害物质,减轻吸烟对人体健康的危害,因此本论文利用固—液吸附理论研究了钴在烟草上的吸附性能以及硒在烟草上吸附的一些影响因素,探讨了吸附质浓度、温度、pH及某些共存物质对吸附的影响,测定了不同温度下的吸附等温线。

We used maize seedlings (Zea mays L.) and tobacco (Nicotiana plumbaginjfolia L.) as material. In the first section, we have investigated cold- shock-induced chilling, drought, heat and salt stress resistance in maize seedlings. In the second section, by modifying the Ca~ and CaM levels, we have tried to establish the involvement of calcium messenger system in the cold-shock-induced cross adaptation. In the third section, we have tried to establish a transgenic tobacco system expression CaMICaMIBP genes, so that the others can use it to investigate the process of physiology and biochemistry which endonuclear and cytosolic calmodulin have taken part in.

以玉米幼苗和烟草为实验材料,本文第一部分通过将玉米幼苗进行短期冷驯化后对冷胁迫、干旱胁迫、热胁迫及盐胁迫的影响,试图证明玉米幼苗中交叉适应现象的存在及其机理;第二部分通过外源修改Ca~(2+)和CaM水平,研究了钙信使系统在交叉适应现象中的可能作用;第三部分通过建立诱导性表达CaM/CaMBP转基因烟草体系,便于研究受细胞质CaM和细胞核CaM调控的各种生理生化过程。

The fungicidal activity of methanol extract was higher than that of ethyl acetate extract, and the inhibition rates were higher than 90% against Alternaria longipes, Fusarium oxysporium, Gibberella zeae, Glomerella cingulata, Exserohilum turcicum under the concentration of 1000g/mL The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, Exserohilum turcicum and Fusarium coeruleum, the EC50 values was 59.4g/mL, 96.5g/mL and 132.5g/mL. In the bioassay of the fermentation products against Bacillus sub tills and Bacillus cereus, by means of detection fo inhibition zone, the inhibition zone diameter was 20mm and 27mm. The results of pot tests that methanol extract showed higher than 70% of protective efficacy and therapeutic efficacy against wheat powdery mildew under the concentration of 2000g/mL.

其中,甲醇提取物的抑菌活性比乙酸乙酯提取物强,在1000g/mL浓度下对烟草赤星病菌、马铃薯干腐病菌、小麦赤霉病菌、苹果炭疽病菌、玉米大斑病菌的菌丝生长抑制率均大于90%;抑制孢子萌发法试验结果表明,2K3菌株菌丝体的甲醇提取物对烟草赤星病菌玉米大斑病菌和马铃薯干腐病菌的EC50分别为59.4g/mL、96.5g/mL和132.5g/mL;管碟法生测结果表明,2K3菌株菌丝体的甲醇提取物对枯草芽孢杆菌和蜡状芽孢杆菌的抑菌圈直径分别为20mm和27mm;盆栽试验结果表明,2K3菌株菌丝体的甲醇提取物在2000g/mL浓度下对小麦白粉病的治疗和保护作用均在70%以上。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好,在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用,抑制率在62.9%~85.1%;抑制孢子萌发试验表明:菌丝体甲醇提取物对烟草赤星病菌的毒力较好,其EC50仅为62.5328μg/mL;抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用,而对大肠杆菌无明显的抑制作用。

PCR and Southern blot analysis confirmed stable integration of the foreign genes into the chloroplast genomes of T_0 and T_1 transgenic plants, and homoplasmy of T_1 transgenic plants.

只转化phaC2基因的叶绿体型转基因烟草基本检测不到有mel-PHAs合成,共表达phaC和phaG基因的叶绿体型转基因烟草则能合成相对较高水平的mcl-PHAs。

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