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We isolate the LCA binding glycosylated proteins from anosia human liver cell extraction and liver cancer cell extraction by LCA coupled epoxy magnetic particles, and the glycosylated fractions were separated by 2D gels which were analyzed by 2D ImageMaster to established disparity expression spectra between Chang Liver and SMMC-7721 firstly.

利用该方法对健康人肝脏细胞Chang Liver总蛋白、肝癌细胞SMMC-7721总蛋白及健康人血清进行了糖蛋白的分离纯化,结合2D电泳技术及其图像分析,建立了健康人肝脏细胞ChangLiver和肝癌细胞SMMS-7721 LCA结合糖蛋白的差异表达谱,分别得到蛋白质点148±5和209±7个,匹配点数为127,匹配率为85.36%。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果:得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱,HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41;组内的平均匹配率分别为91.6%,90.20%对27个差异蛋白质点进行了质谱分析,鉴定出16个蛋白质,在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点;而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

Results showed that the long-term nitrogen fertilization treatment significantly reduced mycorrhizal colonization percentage, arbuscule colonization percentage, number of vesicle and entry points pe.

结果表明长期定位施氮肥显著降低了AM真菌对寄主植物的侵染率、丛枝着生率、单位根长泡囊数和侵入点数

From the cross with P131, 24monoascospore progenies were obtained, 10 of them were avirulent and 14 virulent to Tsuyuake.

因此,将杂交组合产生的杂交后代接种在这4个品种上,以确定稻瘟菌控制对这些品种无毒性的遗传位点数

Chapter 5: The fluorescence quenching reactions of barbaloin with serum albumin in solution were studied. The quenching mechanism of serum albumin by barbaloin was interpreted using the Stern-Volmer mechanism. The binding constant K values and the number of binding sites were calculated, respectively.

第五章:首次研究了芦荟甙与血清蛋白在溶液中的相互作用,利用Stern-Volmer方程解释了芦荟甙对血清蛋白的荧光淬灭,并测定了不同温度下的结合常数及结合位点数

Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites was identified by the absorption spectra.

吸收光谱数据表明,与血清蛋白结合后,噻菁染料单体的吸收峰发生红移,同时强度也有很大变化;还通过吸收光谱计算确定了噻菁染料与血清蛋白的结合位点数

A Theoretical Study on the Reaction Mechanism of Epoxidation Reaction of the 4- Bicyclo

CEPS 思博网--文章书目;-1页数:7 需求点数:28 电子全文:请登入。篇名(4-溴甲基双环[4.4.1]-1,3,5,7,9-十一碳五烯基-3-)甲醇环氧化反应机理的理论研究

Associations between Gene Polymorphisms of CYP1A1, GSTT1 and Frequencies of MNed Binucleated Cells in Residents from an E-waste Dismantling Site

CEPS 思博网--文章书目;-1页数:5 需求点数:20 电子全文:请登入。篇名 CYP1A1, GSTT1基因多态性与电子垃圾处理区居民双核淋巴细胞微核率的关系

Conlusion (1) Decrease of CCK-A receptor binding sites and/or expression of CCK-A receptor mRNA may be a key pathologic factor to damaging cholecystic function in patients of cholelithiasis with liverying insufficiency syndrome.

①CCK-AR结合位点数减少或/和CCK-ARmRNA低表达可能是胆石病肝阴不足证患者胆囊功能损害的重要病理环节。

Assume that the interval [0,1] is countably infinite.

假设区间[0,1]中的点数是可数无穷大的

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。