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Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选，在 PAP基因的 3'端融入了绿色荧光蛋白GFP）基因，然后将融合基因克隆在植物表达载体PBI 121上，再进行遗传转化，得转基因棉花种子5000余粒，将种子播种长到于叶展开时，先在黑暗中用紫外灯照射，查找表现绿色荧光的幼苗，然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交，最后发现有8株棉花表现阳性反应，说明PAP基因的确己经转到了棉花的基因组中，其棉花黄萎病的抗性鉴定正在进行之中。

The control center receives the call from the passenger, then, from a number of red dots appearing on the map on the main monitoring screen, selects the vehicle that is closest to the passenger, and sends a command to the car's built-in mobile communications device.

控制中心接到叫车讯息，便可在监控主机萤幕地图上的诸多红色亮点中，点选距离客户所在地点最近的那部车子，发一通指令到该车的「车机」上，司机收到后按一个键回复确认，就算完成了派车调度的工作。

Now? The control center receives the call from the passenger, then, from a number of red dots appearing on the map on the main monitoring screen, selects the vehicle that is closest to the passenger, and sends a command to the car's built-in mobile communications device.

现在呢？控制中心接到叫车讯息，便可在监控主机萤幕地图上的诸多红色亮点中，点选距离客户所在地点最近的那部车子，发一通指令到该车的「车机」上，司机收到后按一个键回复确认，就算完成了派车调度的工作。

Urban II dolls are a bit the quality of your postings are good, not that ugly day in this handsome, how this can afford that, and we are now graduating from the (05's) junior high school so you do not care about the smaller it Kavalan ah so this is high school so that you are still in high school that day, said a more boring topic so you do not feel tasteless?

1楼市二中的娃娃们你们发贴有点质量好不好，不要一天这个帅那个丑这个玩得起那个又怎么的，我们现在是毕业了（05级的）初中呢么就不计较了噶还小呢本是高中呢啊说呢就是你们了都高中了还在那一天说些无聊呢话题不觉得自己无味吗？

At 3:15 P.M. he got off his own message to Doenitz-- the last radio communication ever to leave the beleaguered bunker in Berlin

下午三点十五分，戈培尔给邓尼茨发了一个电报——这是从柏林被围的地下避弹室发出的最后一封无线电报。

Nuoweiciji, Kyd as well as the Qiao assorted - Howard's three person of groups are the keys which Carlyle relies on, this is we strongest spot, their three people are affirmative send first.

诺维茨基、基德以及乔什-霍华德的三人组是卡莱尔依赖的关键，这是我们最强的点，他们三个人是肯定的先发。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。