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炭疽

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Coli, Shigella sonnei s, Proteus, typhoid and vice Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae and other enteric pathogens are effective; its ethanol extract on the number of gram-positive and-negative bacteria, and human-type Mycobacterium significant antibacterial activity; ebony decoction on in vitro to be sore dermatophytes, Epidermophyton floccosum, plaster-like pathogenic microorganisms in the skin of small spores of fungi

广谱抗菌:实验证明,乌梅水煎液对炭疽杆菌、白喉和类白喉杆菌、葡萄球菌、肺炎球菌等皆有抑制作用,对大肠杆菌、宋内氏痢疾杆菌、变形杆菌、伤寒和副伤寒杆菌、绿脓杆菌、霍乱弧菌等肠内致病菌也有效;其乙醇浸液对一些革兰阳性和阴性细菌,及人型结核杆菌均有显著抗菌作用;乌梅水煎液在试管内对须疮癣菌、絮状表皮癣菌、石膏样小芽孢菌等致病皮肤真菌有抑制作用

By means of mycelium growth rate, the acetone extracts of fermentation products of 39 strain were tested against Botrytis cintrea,Gibberella zeae, Phytophthora capsic, Glomerella cingulata, Bipolaris maydis, Alternaria alternata at the concentration of 500 u g/ml. The results showed that the acetone extract of 1L, 2B and 5J strains fermentation products had a certain degree fungicidical activity.

以番茄灰霉病菌、小麦赤霉病菌、苹果炭疽病菌、玉米小斑病菌和辣椒疫霉病菌为供试菌种,在500μg/ml的浓度下,采用抑制菌丝生长速率法对39株内生真菌发酵产物进行了杀菌活性筛选,发现1L、2B和5J 3菌株的发酵产物丙酮粗提物均对供试病原菌有不同程度的杀菌活性。

The fungicidal activity of methanol extract was higher than that of ethyl acetate extract, and the inhibition rates were higher than 90% against Alternaria longipes, Fusarium oxysporium, Gibberella zeae, Glomerella cingulata, Exserohilum turcicum under the concentration of 1000g/mL The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, Exserohilum turcicum and Fusarium coeruleum, the EC50 values was 59.4g/mL, 96.5g/mL and 132.5g/mL. In the bioassay of the fermentation products against Bacillus sub tills and Bacillus cereus, by means of detection fo inhibition zone, the inhibition zone diameter was 20mm and 27mm. The results of pot tests that methanol extract showed higher than 70% of protective efficacy and therapeutic efficacy against wheat powdery mildew under the concentration of 2000g/mL.

其中,甲醇提取物的抑菌活性比乙酸乙酯提取物强,在1000g/mL浓度下对烟草赤星病菌、马铃薯干腐病菌、小麦赤霉病菌、苹果炭疽病菌、玉米大斑病菌的菌丝生长抑制率均大于90%;抑制孢子萌发法试验结果表明,2K3菌株菌丝体的甲醇提取物对烟草赤星病菌玉米大斑病菌和马铃薯干腐病菌的EC50分别为59.4g/mL、96.5g/mL和132.5g/mL;管碟法生测结果表明,2K3菌株菌丝体的甲醇提取物对枯草芽孢杆菌和蜡状芽孢杆菌的抑菌圈直径分别为20mm和27mm;盆栽试验结果表明,2K3菌株菌丝体的甲醇提取物在2000g/mL浓度下对小麦白粉病的治疗和保护作用均在70%以上。

Objective To study on the security and controlling effect of amino-oligosaccharin for Glomerella cingulata of Aloe vera.

目的2%氨基寡糖素对芦荟炭疽病防治效果及安全性实验。

In the dark during the periods in which experiments were conducted,the infection course of Glomerella cingulata was investigated on time.

将苹果炭疽病菌配成不同浓度孢子悬浮液接种至处于贮藏期的苹果上,在 2 0℃恒温下暗光培养,对病菌侵染过程进行研究。

Bacillus amyloliquefaciens S13 can effectively inhibit Glomerella cingulata. The aim of this study is to investigate the resistant UV light.

液化淀粉芽胞杆菌(Bacillus amyloliquefaciens S13)对檬果炭疽病菌有很好的抑制能力。

Results For Aloe rera,amino-oligosaccharin could control Glomerella cingulata and promote its growth and development evidently,and also proved that amino-oligosaccharin has no harmful effects on Aloe vera.

结果氨基寡糖素能有效促进芦荟生长发育,对芦荟炭疽病有明显的防治作用,安全和无药害发生。

Aloe vera L ; amino-oligosaccharin ; controlling effect ; security ; Glomerella cingulata

库拉索芦荟;氨基寡糖素;防治效果;安全性;芦荟炭疽

Apple ; Qinguan ; Glomerella cingulata ; Storage infection course

苹果;秦冠;炭疽病;贮藏期;侵染过程

C, 200rpm for 5d then placed statically for more than 7d, and addition more than 0.01% Chitosan to Bacillus subtilis suspension provided significant UV protection. The survival spore rate is 90% about 2-2.5 fold better than control for spore exposed to UV radiation for 30 min. Bioassay in against Glomerella cingulata, and the inhibition activity is still 60%.

枯草杆菌於MIY液态培养基中,30°C,200rpm震荡培养五天后静置培养七天以上,以甲壳素添加浓度大於0.01%作为最适宜的紫外保护剂之作用最好,於UVB灯下暴晒30 min,对枯草杆菌孢子的保护效果最好,存活率达90%以上,抗紫外线活性是不添加紫外线保护剂的CK组的2.5-3倍,对檬果炭疽真菌仍有60%的抑菌活性。

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