炭疽

Appressoria were latent in intercellular cleft and were latent until banana fruit were harvested.The development process of conidia of Colletotrichum musae on fruit was not distinct from foliage and stalk.Two pairs of PCR primers were designed according to the two especial fragment (357bp and 206bp) of Colletotrichum musae. Banana tissue culture seedling genomic DNA, banana anthracnose pathogen genomic DNA, mango anthracnose pathogen genomic DNA, rubber anthracnose pathogen genomic DNA,watermelon anthracnose pathogen genomic DNA, banana crown rot pathogen genomic DNA, stylo anthracnose pathogen genomic DNA, watermelon Fusarium wilt pathogen genomic DNA were extracted using SDS method.

根据香蕉炭疽菌的两个特异片段(分别为357bp和206bp)，设计两对引物：采用SDS法分别提取了香蕉组培苗基因组DNA、香蕉果实炭疽菌弱致病株Z_1基因组DNA、香蕉果实炭疽菌强致病株Z_4基因组DNA、芒果炭疽菌基因组DNA、橡胶炭疽菌基因组DNA、柱花草炭疽菌基因组DNA、西瓜炭疽菌基因组DNA、香蕉冠腐病菌基因组DNA、西瓜枯萎病菌基因组DNA；以上述基因组DNA为模板对特异片段进行PCR验证，证明357bp的片段为Colletotrichum musaes所特有，可以用此片段进行香蕉果实炭疽病的分子检测试验，。

Based on various testimonies including the 12 confessions at Khabarovsk Trial, Unit 731 based in Harbin and Unit 100 based in Changchun, besides using living humans as subjects in their experiments on anthrax and glanders as documented in the autopsy reports in Dugway Library, both mass produced anthrax. Unit 100 also mass produced glanders. Unit 731's monthly production capability of anthrax is 600kg. From 1941 to 1942, Unit 100 produced 1000kg of anthrax and over 500kg of glanders.

据包括以上12名以外的日军细菌部队有关人员供述：驻哈尔滨的731部队和驻长春的100部队，除了用活人做实验研究炭疽和鼻疽以外（参考以上提到美军达格威试验场技术资料馆保存的日军提供的有关人体实验解剖报告），还大量生产炭疽菌，100部队还大量生产鼻疽菌。731部队一个月的炭疽菌生产能力达约600公斤；1941年到1942年间，100部队生产了1000公斤炭疽菌、500多公斤鼻疽菌。

C. gloeosporioides mycelium grew well on media with fructose and glucose as carbon source while sorbose and starch promoted sporulation. The mycelia of C. dematium grew well with maltose as carbon source while starch promoted sporulation. Of the nitrogen sources tested, organic nitrogens were beneficial for both mycelial growth and sporulation but chemical nitrogens suppressed both growth and sporulation.

供试碳源中果糖和葡萄糖有利于胶孢炭疽菌的菌丝体生长，山梨糖和淀粉有利于其产孢；麦芽糖有利于黑线炭疽菌的菌丝体生长，淀粉有利于其产孢；氮源中有机氮有利于2种炭疽菌生长和产孢，化学氮源则表现抑制作用。

The progress of studies on Stylosanthes in the aspects of stylo anthracnose caused by the fungus Collectotrichum gloeosporioides and its genetic diversity and epidemiology, breeding, cytogenetics, cytobiology, tissue culture, protoplasm fusion, gene clone, genetic transformation, classification and molecular makers was reviewed in this paper.

摘要综述了柱花草的炭疽病危害、炭疽病原菌的遗传多态性及流行性、柱花草的抗炭疽育种、细胞遗传学、细胞生物学、柱花草组织培养、原生质融合、基因克隆、遗传转化、柱花草分类、分子标记、航天辐射育种等方面的研究进展。

The reaction of detached leaves Stylosanthes of to Collectotrichum gloeosporioides was tested. The stylo leaves of Mineirao were hypersusceptible in seven days, while stylo leaves of CIAT2312 were rotted in the same time.

对这2种柱花草的离体叶片接种炭疽菌，Mineirao柱花草叶片对炭疽菌发生过敏性反应，而CIAT2312柱花草叶片则腐烂，表明Mineirao柱花草叶片中可能含有抑制炭疽菌的物质。

Chestnut is a high valued economic orchard crops in Guangdong mountain area. A new disease on the leaves of chestnut was found in chestnut orchard of SCAU and areas where chestnut grows in Guangdong. Its symptom was different with those any reported leaf disease on chestnut. The pathogen was identified as Colletotrichum gloeosporioides, and is the same as the pathogen of Coniella castanicola, anthracnose of Camellia oleifera and Caryota ochlandra.

近年，在华南农业大学板栗园及广东一些板栗产区，栗叶上出现了一种新的病害，其发病症状与已报道的板栗叶部病害无一是完全符合的，经鉴定，这种病原菌属于半知菌亚门，腔孢纲，黑盘孢目，黑盘孢科，刺盘孢属，胶胞炭疽菌Colletotrichum gloeosporioides，与板栗炭疽病、油茶炭疽病以及鱼尾葵炭疽病的病原一致。

The result showed that the optimum temperature of mycelium growth was 28 ℃ for Colletotrichum gloeosporioides and Colletotrichum acutatum.

通过对2种橡胶炭疽病菌的生物学特性进行研究，结果表明：胶孢炭疽和尖孢炭疽的最适温度均为28℃。

However, the alternation of light and darlmeS5 every 12 hours was found to be the best for Colletotrichum acutatum.

它们对碳源的利用有较大差异，胶孢炭疽在麦芽糖上生长最好，而尖孢炭疽在甘露醇和乳糖上生长较好。2种炭疽菌对甲基托布津的敏感性差异显著，尖孢炭疽有较强的耐药性。

The biological characters of Colletotrichum gloeosporioides and Colletotrichum acutatum was reported in this paper.

和尖孢炭疽菌(Colletotrichum acutatum Simmonds)均能引起橡胶炭疽病的发生，胶孢炭疽菌是主要病原。

Provided as a public service by Office of the Assistant Secretary of Defense-Public Affairs and the Anthrax Vaccine Immunization Program Agency, the AVIP web site reports information on anthrax including: the threat from anthrax, anthrax infection, anthrax vaccine and vaccination protection.

这是炭疽杆菌疫苗免疫项目的网站。它是由公共防御事件辅助办公室和美国炭疽杆菌疫苗免疫项目局共同创建并服务于大众，提供有关炭疽杆菌的信息，如：来自炭疽杆菌的威胁、炭疽杆菌感染、炭疽杆菌疫苗以及疫苗保护。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。