灵敏度分析

Chemiluminescence analysis has the virtue of highly sensitivity,broadbrimmed linear range and quick analysis speed.

针对化学发光分析灵敏度高、线性范围宽、分析速度快等优点，介绍了近年来化学发光新体系在药物分析中的应用研究进展。

The theory of rigid column receiving capability, the principles of acceleration sensor, the characteristic parameters of vector hydrophone and the relationships ...

主要包括刚硬柱的接收性能理论分析，加速度计的工作原理分析，矢量水听器特性参数的分析，声场中各灵敏度关系的分析等。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Firstly, the relevant features of three kinds of structure such as CMSA, PMSA, FMSA are summarized and compared ,and the advantages of finger-shaped variable-capacitance micromechanical accelerometer on the base of bias stationary structure are presented.

并以该结构为分析对象，从加速度计的分辨率、量程、稳定性、灵敏度等性能分析比较了多种结构梁，提出了折叠梁的综合性能最好，最后通过分析计算出了折叠梁在检测方向的刚度。

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

One way to speed up an LC run is to increase the temperature, and this article discusses the benefits of elevated temperatures, including sensitivity improvements, selectivity changes and simultaneous analysis of polar and nonpolar analytics using temperature gradients.

一种提高分析速度的方法是提高温度，本文讨论了升高温度的优势，包括灵敏度改善，选择性变化，以及采用温度梯度同时分析极性和非极性分析物。

Activation analysis and non-radiometric method have been used to measure long-lived fission product nuclides. Their sensitivity is superior to that of traditional radiochemical analysis.

活化分析以及非放射的仪器分析方法被用来测量长寿命裂变产物核素，其分析灵敏度优于传统的放化分析法。

The measurement system error model analysis is introduced into power system real time network state analysis as an important part for the first time, which can improve on the state estimation quality and provide the capability to monitor the operation of the measurement system; 2. The theory and algorithm of the on-line estimation and update of measurement noise variance based on the relation between the residual variance and noise variance. The statistic properties of the sample variance are discussed and the relation between the estimation precision and sample size under given confidence level is derived; 3. The theory and algorithm of detection and identification of measurement bias are presented, which is based on the relation between residual mean and noise mean. The statistic properties of sample mean are discussed and the relation between estimation precision and sample size is derived; 4. The Givens orthogonal transformation algorithm is selected to be the essential algorithm of state estimation, the fast orthogonal transformation algorithm with damp factor and the algorithm which can handle the zero injection measurements efficiently are presented; 5. The quantity analysis theory of bad data detectivity and identifibility are presented, which describes the relation between the elements in matrix W〓 and bad data amplitude and can provide the theory base for measurement system design and valuation.

一、首次将量测系统误差模型分析做为一个环节引入电力系统实时网络状态分析中，为EMS系统增加了实时监视系统运行、修正量测系统误差模型的新功能，进一步发挥了实时网络状态分析应用软件的潜力；二、首次提出了应用样本方差在线估计与修正量测系统误差方差的基本理论，讨论了样本方差的统计性质和概率分布，推导出了样本容量、估计精度和置信度之间的关系，给出了在线估计与修正量测系统误差方差的算法；三、首次提出了应用样本均值在线检测与辨识量测偏差的基本理论，讨论了样本均值的统计性质，推导了样本容量、估计精度和置信度之间的基本关系，给出了在线检测与辨识量测偏差的算法；四、在状态估计算法设计中，以Givens变换算法做为基本算法，提出了快速正交变换阻尼因子法和可以有效地处理零注入量测的混合法，并对实时应用中的一些问题进行了讨论；五、提出了不良数据可检测性与可辨识性的定量分析理论，揭示了描述量测系统配置、网络结构与参数的残差灵敏度矩阵中的元素与不良数据的幅值在可检测、可辨识能力上的定量关系，为量测系统配置设计与评价提供了理论基础；六、综合国内外最新研究成果，采用自适应自回归预测技术和稀疏矢量技术，构造了较完善的不良数据检测与辨识算法。

The cylindricity could be calculated by least squares method and wavelet error separation method that the measurement error could be decomposed by Harmonic analysis algorithm based on wavelet theory.

针对激光 CCD 灵敏度高，干扰噪声容易混入测量数据中的问题，提出了一种基于小波分析的误差分离方法，即运用谐波分析的思想方法和小波理论中多频率分析算法进行误差分离，误差分离后的数据用最小二乘法处理求出圆柱度。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。