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To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn, TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1,建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系,并对体系进行优化。

RESULTS Using titrimetric method of periodic acid is a shortcut and convenient method.

结果 高碘酸滴定法作肌苷含量测定,方法简便快捷,终点变色灵敏,测定结果准确可靠。

A new tribasic complex was found and used to explain that bismuth thiocyanate is a sensitive colour reaction in the Tween 80 H 2O solution.

发现形成一种新的三元配合物,并用来解释在吐温-80水溶液中硫氰酸铋为一种灵敏的显色反应。

The FosB/△FosB positive staining was detected in nucleus accumbens septum, tuberculum olfactorium and dorsal caudate putamen, without little cFos positive staining. The results indicate the expression of cFos and FosB/△FosB display the differences in area and intensity, and the area intensively expressing FosB/△FosB is more nearly coincide with the area related to schizophrenia. So FosB/△FosB is a better mark to research the pathogenesis of schizophrenia.

同时大量研究证明,动物模型的行为及生理变化与中枢神经系统神经元的病理变化有关,因此,利用即早基因反应的灵敏性、功能多样性、检测方便性等,应用免疫组织化学的方法对模型小鼠脑内cFos和FosB/△FosB蛋白的表达进行检测,通过确定不同脑区cFos和FosB/△FosB蛋白的表达,来研究哪些脑区或神经元参与了反应,从而发现精神分裂症在脑区内所涉及的解剖结构,有助于对精神分裂症发病机制的认识。

The method of turbidimetric immunoassay can be applied on automatic analyzer for detection of RBP in the serum.

结论免疫透射比浊法测定血清视黄醇结合蛋白,方法简便、快速、灵敏,结果准确,可用自动分析仪测试,适合临床检验科应用。

A series of experiments were done to substantiate the feasibility of the different piezoelectric cochlea implants in vitro and in vivo . The unpoled ceramic had no piezoelectric characteristic and no ability to decrease the threshold, while the poled ceramic had obvious piezoelectric effect to recover the hearing. Based on these results, it can be affirmed that the piezoelectric cochlea implant can activate the nerve and decrease the threshold.

本研究通过未极化与极化的高灵敏性压电陶瓷的对比,从体外模拟实验中的声电响应曲线和动物体内植入实验的结果中得到:未极化的压电陶瓷没有压电响应,而极化后的压电陶瓷能够将声音信号转化为电信号,证实了压电陶瓷的压电特性的确可以刺激动物的听神经,从而降低听阈阈值。

A series of experiments were done to substantiate the feasibility of the different piezoelectric cochlea implants in vitro and in vivo. The unpoled ceramic had no piezoelectric characteristic and no ability to decrease the threshold, while the poled ceramic had obvious piezoelectric effect to rec

本研究通过未极化与极化的高灵敏性压电陶瓷的对比,从体外模拟实验中的声电响应曲线和动物体内植入实验的结果中得到:未极化的压电陶瓷没有压电响应,而极化后的压电陶瓷能够将声音信号转化为电信号,证实了压电陶瓷的压电特性的确可以刺激动物的听神经,从而降低听阈阈值。

There were not obviously interference to vanadic acid oxidation method when TG≤8.0 mmol/L, HB≤6.0 mmol/L.

钒酸氧化法测定胆红素准确、灵敏、稳定,线形范围宽,是测定胆红素的理想方法。

The optimal parameters for catalytic reaction of LiP or MnP on theelectrode in electrochemical enzyme sensor are obtained,which are pH of4.2,H_2O_2 concentration reaching 0.1875 mM,0.25 mM hydroquinone and 6mM veratryl alcohol.In the optimal reaction system,LiP and MnP aredetermined exactly by the sensor.

实验确定了电化学酶传感器裸玻碳电极对LiP、MnP体系的催化反应的响应最为灵敏且电流变化较大的最适pH值(pH4.2)、H_2O_2(0.1875 mM)、对苯二酚(0.25 mM)、藜芦醇(6 mM)含量等参数,在该反应条件下传感器能准确测定LiP、MnP。

3Log.Conclusion:The method of trace TCID50 was objective,sensitive and handy in evaluating the virucidal effect of disinfectants.

用微量TCID50法评价消毒剂对病毒的灭活效果,客观、灵敏、简便易行

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。