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The dynamic changes of endothelin-1 in cerebrospinal fluid , brain tissues and plasma and ET〓-RNA expression in hypothalamus, hippocampus and temporoparietal grey and white matters were observed by radioimmunoassay methods and spot hybridization respectively.

四、建立双后肢高速、低速投射物致伤犬应激模型,应用核酸斑点杂交和放射免疫分析法,检测分析致伤前及致伤后10min、1h、2h、6h、8h,对犬的脑组织、血浆、脑脊液中的ET〓(内皮素-1)含量及海马、丘脑下部、颞顶叶灰、白质中ET〓-RNA表达的动态。

The results were as follows: ET〓-RNA expression levels in hypothalamus, hippocampus and temporoparietal grey and white matters of the low and high speed injury groups were elevated significantly than that of control group (p<0.05), but in the above areas of brain, ET〓-RNA expression in hypothalamus and hippocampus were the highest (p<0.01), especially in the high speed injury group; ET〓 contents in hypothalamus and hippocampus in the two injury groups, especially in the high speed injury group, increased very significantly than that of control group (p< 0.01); ET〓 contents in CSF and plasma in the two injury groups, also especially in high speed injury group, increased markedly after 2 hours, and lasted for 8 hours after injury; ET〓 contents in the abovt areas of brain were of highly positive correlation with that in plasma or in CSF.

实验显示:致伤犬伤后海马、丘脑下部、颞顶叶灰、白质内皮素—1RNA表达较对照组显著增强(p<0.01,p<0.05),其中以海马和丘脑下部最显著(p<0.01),高速组最明显(p<0.01);海马和丘脑下部ET〓含量较对照组显著升高(p<0.01);血浆、CSF中ET〓含量在伤后2h即极显著升高(p<0.01),并持续至伤后8h,上述各组均以高速组最为显著(p<0.01);各脑区和血浆,CSF中ET〓含量间呈显著正相关。

The effect of the overlaying tiss ue on the measurement can not be ignored because the most interested tissue to b e monitored is the deeper tissue layers,such as muscle and brain,which are under adipose or skull.Apart from this,variations of the regional hemoglobin oxygen s aturation (rsO2),haematocrit and regional blood volume hav e their influences on the measurement.

然而在人体组织这个复杂的不均匀混合介质中,深层组织往往是感兴趣的待测部位,如皮脂下的肌肉组织,头皮和颅骨下的脑灰白质等,这时外层覆盖组织的存在会直接影响检测的精度;另外,全血的局部氧饱和度( rsO2)的波动,红细胞比容的变化,以及血容积都对测量精度产生影响。

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