灭活

Pseudosciaena crocea were immunized with formalin-killed and heat-killed trivalent vaccines by immersion and injection, which was made up of four experimental groups.

采用0.5%福尔马林4℃灭活和65℃热灭活2种不同的方法制备三联疫苗作为抗原，以多次腹腔注射或浸泡的方式免疫大黄鱼，通过测定受免大黄鱼血清中抗体凝集效价变化并进行攻毒实验以评估保护效果。

In vivo, composition E markedly protected mice from lethal challenge by heat-killed E. coli and heat-killed S. aureus. Conclusions:(1) Targeting on PGN, CpG DNA and lipid A, we successfully established the platform to screen anti-inflammatory traditional Chinese medicine using biosensor technology which is highly effective, objective and feasible.

该组分经MTT法测定对小鼠RAW264.7细胞活力无影响(≤400μg/ml)，经CCK-8法测定也对小鼠RAW264.7细胞活力无影响(≤800μg/ml)；对PGN、CpG DNA及LPS刺激小鼠RAW264.7细胞分泌TNF-α有显著的抑制作用，并呈现良好的剂量-效应关系；对致死剂量的热灭活大肠杆菌和热灭活金黄色葡萄球菌攻击小鼠有显著的保护作用。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

A field trial was continued, 85, 916 pullets of ten flocks in five farms were vaccinated with our preparation.

我们在完成了研制的EDS一76灭活油乳剂苗和国内外同类产品的免疫对比试验，取得了较好结果后，继续进行EDS一76灭活油乳剂苗的免疫保护性试验。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病(本论文仅供参考，如需转载本文，请务必注明原作者以及转载来源：论文图书馆 www.lwlib.com)作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

moxa fumigation in ward had a certain inactivation effect to HBsAg and HBeAg,but didn't completely inactivate HBV,didn't replace disinfectants on inactivation to virus,and may be used in air disinfection of baby-friendly ward.

为了进一步弄清艾条熏蒸对病毒是否有灭活作用，我们按有关文献方法［3，4］，在爱婴区通过艾条熏蒸对乙肝病毒HBsAg和HBeAg抗原性的灭活进行了研究，现将结果报告如下。

By chicken embryo, minimum concentration of medicine of directly inactivated test、 treatment test and preventive test is 7.5mg/ml、 62.5mg/mland 31.25mg/ml;By CEF, minimum concentration of resiting NDVF_(48)E_8 and moderate virulence strain is 10mg/ml、 50mg/ml and 25mg/ml. However,the result of complex prescriptionpreventing and curing NDVF48Ej$ is not ideal.

在鸡胚上抗病毒试验中，对三株病毒起直接灭活、治疗和预防作用的最低药物浓度分别为7.5mg/ml、62.5mg/ml和31.25mg/ml；在鸡胚成纤维细胞上抗病毒试验中，对强强毒株和中等毒力株起直接灭活、治疗和预防作用不引起细胞病变的最低药物浓度分别为10mg/ml、50mg/ml和25mg/ml。

Second, procedures of effective protoplasts fusion and U.V. irradiation or heat treatment protoplasts fusion were developed. Equal protoplast suspensions (containing about 108/ml protoplasts) of each strain were mixed and washed by centrifugation, then the pellets were resuspended in PEG4000 solution (35%) at 37癈 for 2min.

其次，确定了原生质体融合的基本条件及灭活原生质体融合的条件：等量混合两亲株原生质体或用热灭活的原生质体(＞10~8个／ml)，用35％的PEG4000在37℃处理2min后，稀释，涂布再生，可获得融合率在50％左右的融合子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。