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灌洗

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Bronchoalveolar lavage and wright staining analysis showed that thetotal number of the all inflammatory cells in asthma model group andthe normal control were (36.2±4.6)×10~6/ml and (9.5±1.6)×10~6/ml.There was significant difference between the two groups (p<0.01).Meanwhile, the number of acidophilic cell, macrophage andmacrophage were also increased in asthma model group rather thanneutrophilic granulocyte.

支气管肺泡灌洗和细胞记数显示,哮喘模型组支气管肺泡灌洗液细胞总数为(36.2±4.6)×10~6/ml,与正常对照组(9.5±1.6)×10~6/ml,相比显著增加(p<0.01),同时嗜酸性细胞、巨噬细胞和淋巴细胞增加明显,而中性粒细胞变化不明显。

The rabbits' RNA was extracted from the lavage cells, and mRNA for TNFα was quantitated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase as an internal standard.

第二部分:12只表面活性物质缺乏兔,以CMV(n=6)或PHY+PEEPi(n=6)通气1h后,重复灌洗,提取灌洗液中的兔RNA,以GAPDH为内参照RT-PCR的方法来定量分析TNFαmRNA。

We did bronchovesicular lavage with 10 mL normal saline, then collected all cells in BALF, stained cells by Giemsa's stain and counted the total number of cells and differential cells numbers for observing the inflammatory changes in lungs after RSV infection.

以10 ml生理盐水进行支气管肺泡灌洗,收集肺泡灌洗液中细胞,常规吉姆萨染色,行细胞分类计数,观察RSV感染对肺脏的炎症影响。

Methods: 60 cases of chronic severe hepatitis were divided into observe group and control group randomly, 30 cases in respectively. Control group was treated with routine approaches comprised of liver protecting, kata-ALT, detoxicating and albumin supplementing, et al, at the same time, adding colon herbs dialysis to the cases of observe group with HC-3000 therapeutic machine. At first, intermittence lavage using coloclysis solution at the depth of 50cm, 60min, then infund 100~200ml herbs colon dialysis solution constituted by rhubarb, acori graminei, semen raphani, dandelion, oyster and cornu bubali, at al, remaining 1~2h, biw, continuous 4wks. Observe the amelioration of primary symptoms, physical signs and complications in all of the cases, as well as the change of liver function, prothrombin time and the contents of blood ammonia.

60例慢性重型肝炎患者按分层随机配对分为观察组和对照组各30例,对照组给予常规护肝、降酶、退黄以及补充白蛋白等支持治疗,观察组在基础上加用HC-3000结肠途径治疗机透析治疗,每次先用结肠灌洗液间歇灌洗60min,深度50cm,然后注入由大黄、石首蒲、莱菔子、蒲会英、牡蛎、水牛角等组成的中药结肠透析液100~200mL,保留灌肠1~2h,每周2次,连续4周后观察患者主要症状、体征及并发症的改善情况,同时检测治疗前后肝功能(TBil、ALT、ALB、CHE)、凝血酶原时间和血氨(NH3)等指标。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组,以体外直肠不全结扎法造模,以动态浊度法检测各组大鼠血浆内毒素水平,放射免疫分析法检测各组血清TNF-α含量;②大鼠处死后,取左肺进行支气管肺泡灌洗,以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平;③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数,计数兰染的细胞数,求出死亡百分率;在光镜下计数BALF沉渣200个细胞,计算中性粒细胞所占百分比。

Results:The following phenomena were observed:the Ph, PaO2 of the model rabbits descended sharply, the PaCO2 increased lightly, the HR slowed down obviously, but the BR increased, the SBP and DBP of the model rabbits descended markedly, and the PS content decreased dramatically. The pneumonic pathology changes showed pneumorrhagia, atelectasis, shrink of alveolus and pulmonary hyaline membrane. The above results indicated that it accorded with the standard of I-type of acute respiratory distress syndrome.

结果:整肺灌洗后模型兔的血气pH、PaO2急剧下降,PaCO2 轻度上升,心率明显减慢而呼吸频率增加,SBP和DBP均明显下降;支气管肺泡灌洗液中PS含量明显下降;病理检查提示出现肺出血、肺不张、肺泡萎缩、透明膜形成等病变,符合Ⅰ型重症呼吸衰竭临床诊断标准。

First of all, with aluminum silicate, or cloth bleaching earth (Fuller's earth) or gastric tube every 2 hours to 60 grams of activated carbon for gastrointestinal lavage; hemoperfusion adsorption can be used following the poison can often save critically ill patients.

首先用矽酸铝,或漂布土(Fuller′s earth)或经胃管每2小时给活性炭60克作胃肠灌洗;继可用血液灌流吸附毒物往往能挽救重危患者。

They were given brocho-alveolar lavages with the saline which include antibiotics, cortical hormone and other drugs and mechanical ventilation were also used when necessary.

方法对28例老年慢性阻塞性肺疾病急性加重并严重呼吸衰竭患者应用纤维支气管镜诱导经鼻气管插管,并进行肺泡灌洗(采用配有抗生素、激素等药物的生理盐水进行分次灌洗治疗),连用机械通气。

After 1hour,4 and 6hours,bronchoalveolar lavage was done,and the function of PAMs,the amount of PAMs and the lung relative weight were determined.Results Lycopene could increase the phagocytic function of PAMs significantly .

以05,10,30mg/的番茄红素连续给雄性Wistar大鼠灌胃30d,用脂多糖制造大鼠急性肺损伤模型,分别在1,4,6h后支气管肺泡灌洗,检测肺泡巨噬细胞吞噬功能、细胞计数以及肺相对重等指标。

Methods Male rats were gavaged continuously by lycopene with 0 5,1 0,3 0mg/kg for 30 days;Lipopolysaccharide was used to make the model of ALI rats.After 1hour,4 and 6hours,bronchoalveolar lavage was done,and the function of PAMs,the amount of PAMs and the lung relative weight were determined.Results Lycopene could increase the phagocytic function of PAMs significantly .

以0 5,1 0,3 0mg/的番茄红素连续给雄性Wistar大鼠灌胃30d,用脂多糖制造大鼠急性肺损伤模型,分别在1,4,6h后支气管肺泡灌洗,检测肺泡巨噬细胞吞噬功能、细胞计数以及肺相对重等指标。

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