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Methods Ten rats were subjected to anatomy following caoutchouc perfusion into the artery by inserting tubes through the heart, 10 were subjected to dissection following artery and vein perfusion, and 10 were subjected to dissection immediately following anesthesia.

采用经心脏插管天然橡胶灌注方法对10只大鼠行动脉灌注后解剖、10只大鼠行动静脉同时灌注后解剖, 10只活体大鼠麻醉后直接解剖,观察大鼠尾动脉情况并测量尾动脉不同平面的外径。

Methods Ten rats were subjected to anatomy following caoutchouc perfusion into the artery by inserting tubes through the heart, 10 were subjected to dissection following artery and vein perfusion, and 10 were subjected to dissection immediately following anesthesia. Diameter of the caudal artery was measured.

采用经心脏插管天然橡胶灌注方法对10只大鼠行动脉灌注后解剖、10只大鼠行动静脉同时灌注后解剖, 10只活体大鼠麻醉后直接解剖,观察大鼠尾动脉情况并测量尾动脉不同平面的外径。

Methods: Myocardial ischemia-reperfusion injury model was established through accluding the left anterior descending branch of coronary artery for 60 min and removing the ligation tater to reperfuse for 30 min.

结扎大鼠冠状动脉左前降支60min,再灌注90min复制大鼠心肌缺血再灌注损伤模型,观察心安灵对大鼠心肌缺血再灌注损伤的保护作用。

Methods: Rat infrarenal AAA models were created via infusion . Porcine pancreatic elastase was infused in treated group and saline was infused in control group .The change of NF-κB was detected by immunohistochemistry and was analyzed by computer image analysis.

采用灌注法制备大鼠AAA模型,实验组采用猪胰弹力酶灌注,对照组采用生理盐水灌注,免疫组化法观察NF-κB的阳性变化,计算机图像分析仪分析其变化规律。

Materials and Methods: Two cases of brain tumors (one was glioblastoma, the other was central neurocytoma) were performed dynamic enhanced CT and DSC-T2~* MR perfusion imaging before operations.

材料和方法:术前对2例脑肿瘤(1例胶质母细胞瘤,另1例中央神经细胞瘤作为对照)分别行动态对比增强CT灌注和动态T2~*敏感对比增强平面回波(DSC-T2~*)MR灌注成像,分析两种灌注方法的rCBV、CBF结果。

This experimental study, based on the ischemia-reperfusion injury model of the isolated rabbit liver, was to investigate hepatocellular apoptosis and its mechanism during cold preservation-reperfusion of donor liver and assess the structural and functional damages of the reperfusing donor liver subsequent to rewarm ischemia. In addition, the expression and effects of ICAM-1 mRNA were mainly observed during rewarm ischemia-reperfusion of donor livers. Our experiment included three parts: The first was establishment of the isolated liver ischemia-reperfusion injury model in rabbits.

本实验在建立离体供肝缺血-再灌注损伤模型的基础上,研究供肝低温保存-再灌注过程中肝细胞凋亡现象及其相关机制;探讨作为临床肝移植中供肝的另一缺血阶段—复温缺血对再灌注后供肝结构及功能的损伤作用,并着重观察供肝复温缺血-再灌注期间组织细胞内细胞间粘附分子-1 mRNA的表达规律及其作用。

The perfusing method improved with new technique is quicker and more effective than the traditional one and results in the successful rate improvement.

改进的灌注方法简单实用,既缩短了灌注时间、热缺血时间和手术时间,又减轻了缺血再灌注损伤,提高了成功率。

Rats were randomized into an experimental group (n=7), in which limbs were selectively perfused with a cooled isotone heparin solution at a limited flow rate before blood flow was restored, and a conventional group (n=7; uncontrolled blood reperfusion).

将上述7只大鼠随机分配到试验组中,在血流量恢复前,大鼠肢体选择性灌注冷却的等渗肝素溶液并限制灌注流速。常规组(7只大鼠)采用非控制性血液再灌注。

The results show that the temperatures on the surface of the tongue rise with increasing of the blood perfusions.

结果表明,舌表面温度随血液灌注率的增加而升高,但血液灌注率增大到一定值后,舌面温度将维持不变,血液灌注率值也不再增长。

The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血/再灌注模型,健康SD大鼠24只,随机分为心包打开假手术组(A组,n=6),缺血/再灌注对照组(B组,n=6),5 mg·kg~(-1)氯胺酮+缺血/再灌注组C组,n=6,缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮,10mg·kg~(-1)KTM+I/R组D组,n=6,缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10%水合氯醛40 mg·kg~(-1)腹腔注射麻醉,气管切开,连接多功能监护仪记录心电图;小动物呼吸机人工呼吸,呼吸频率60次/分,潮气量2ml/100g,于左胸第四肋间打开胸腔暴露心脏,在左心耳下1mm左冠状动脉处,用丝线,眼科外用不锈钢小圆针穿过心肌浅层,稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎,B、C、D组结扎);结扎开始左心室心尖部即由红色变暗,30 min后呈暗红色,心电图中出现S-T段抬高,说明缺血形成。

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