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Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化;并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组(n=10)及葛根素组(n=10),所有动物均阻闭腹主动脉肾下段20min,建立兔脊髓腰尾段缺血模型。

Methods A pathological model of cerebral ischemia reperfusion damage in rats without craniectomy was developed referring to Koizumi and Longa's method.

参照Koizumi和Longa's法建立大鼠不开颅的脑缺血一再灌注损伤病理模型。

Reversible middle cerebral artery occlusion without craniectomy in rats[J].

红景天苷对大鼠局灶性脑缺血再灌注损伤的保护作用[J]。

METHODS: The expression of Bax was detected by flow cytometer in cortical neurons under ischemic like conditions.

利用流式细胞仪对小鼠皮质神经元类缺血再灌注损伤后Bax的表达进行检测。

METHODS: The expression of Bax was detected by flow cytometer in cortical neurons under ischemiclike conditions.

利用流式细胞仪对小鼠皮质神经元类缺血再灌注损伤后Bax的表达进行检测。

Cytophaga sp was selected for heparinase production because it was more productive. Three heparinases, named HⅠ, HⅡ and H Ⅲ were purified from the crude cell extract by means of batch DEAE-Cellulose negative adsorption, Hydroxylaptide adsorption and releasing, SP-Sepharose cation exchange chromatography, Source 30S cation exchange chromatography, Poros 20HS cation exchange perfusion chromatography and Sephacryl S200HR gel permeation chromatography.

利用超声破碎、DEAE-纤维素反吸附去杂、羟基磷灰石批处理吸附层析、SP-Sepharose离子交换层析、Source 30S离子交换层析、Porous20HS离子交换灌注层析及Sephacryl S200HR凝胶排阻层析,从噬纤维菌中纯化出三种新的肝素酶。

Objective To study the cytotoxic effect of thiotepa and mitomycin C on human bladder cancer cell line in vitro.

目的 探讨体外膀胱灌注噻替哌和丝裂霉素C药物浓度和作用时间对膀胱癌细胞杀伤作用的影响。

The slip knot was tightening by the drawn-string through the cardiac catheter which can be removed after decannulation. The modified Dennis cannulation was done with left ventricular assistance from the left atrium (via right external jugular vein) to the right subclevian artery in 6 dogs.

这种方法用于从右颈外静脉到左心房插引流管,和从右锁骨下动脉插灌注管行左心辅助的试验犬6例,在拔管后房间隔插管处可被闭合。

The results of this study suggested that different activated sludges could tolerate the concentration of 25% NaCl by the acclimatized procedure and decolorize the simulated dye wastewater with high salt concentration in anaerobic conditions.

食品、纺织、染料、制药和化工/石油等行业,在生产过程中产生大量的含盐废水,含盐量有时高达15%~20%[2,3]。目前,对此类废水一般采用电解法、膜分离法、焚烧法和深井灌注法等技术处理。

Histological examination showed that nm-haFGF markedly attenuates the renal edema,brush border's defluvium and cell necrosis induced by ischemia-reperfusion.

组织学检查结果显示,nm-haFGF可明显减轻缺血再灌注引起的肾组织水肿,肾小管刷状缘脱落和细胞坏死。

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