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At each time point, we observed morphological changes, in situ detected DNA breaks, and observed expression changes of production of lEGs c-Fos and DNA repair enzyme APE/Ref-1 which participated in many kinds of DNA repairs. Results in the experiment were shown as follow:DHE stain indicates: The model of rat MCAO/R was credible, and the damaged regions included cortex (lobus frontalis, lobus arietalis, lobus occipitalis et al and caudatum.

本次实验采用大鼠大脑中动脉阻塞再灌注模型,将健康雄性SD大鼠随机分为正常组、假手术组和MCAO2h再灌注2h、6h、12h、24h、48h、72h组,观察各时间点损伤区域神经元和细胞的形态学变化,原位检测DNA断裂损伤,检测DNA修复酶APE/Ref-1表达变化情况以及IEGs产物c-Fos表达变化情况。

At endpoint, every cardiac function parameter in group MSCs and TXL was not significantly different compared with group control (all P>0.05), however, the number of dyskinetic segments (P=0.039) and infarcted size (P=0.0061) in group simvastatin, and the number of dyskinetic segments (P=0.04) in group atorvastatin, were remarkably decreased compared with group control.

终点时,MSCs组、通心络超微粉组和对照组无显著差异(47.3±13.2%,44.8±13.9%vs 47.8±11.1%,P>0.05),而辛伐他汀组和阿托伐他汀组灌注缺损面积均小于对照组(39.3±12.4%,38.7±13.1%vs47.8±11.1%,P<0.05);尤其是通心络超微粉+MSCs组、辛伐他汀+MSCs组、阿托伐他汀+MSCs组的灌注缺损面积进一步显著减小(27.6±7.4%,28.3±7.7%,29.3±9.0%vs 47.8±11.1%,P<0.05)。

Results Both doses of SM and bFGF pretreatment before I/R, decreased the MDA, increased the activities of SOD and GSHpx, evocate levels of GSH and NO.

结果 bFGF和SM联合预处理组心肌中SDH、SOD、GSHpx活性明显高于缺血再灌注组,抗氧化物GSH和NO的含量明显高于缺血再灌注组,心肌病理改变明显好于未用bFGF和SM组。

Multislice CT perfusion imaging provided a new imaging method for measuring the uterine hemadynamic.

子宫多层螺旋CT灌注成像技术方法可靠,为定量检测子宫血流灌注状态提供了一种新的影像学方法。

Objective The present study is to explore the safe of chemotherapeutic hyperthermic intraperitoneal perfusion by using the HGGZ-102 hyperthermic perfusion machine.

目的 探讨HGGZ-102型体腔热灌注治疗机对犬腹腔热灌注化疗的安全性。

METHODS:Hind limbs ischemia was made by clamping infrarenal aorta with a microvascular clip and lung injury was made by following reperfusion. Lung tissue was obtained from the animals subjected to sham operation, 4 h ischemia without reperfusion and 4 h, 8 h, 16 h, 24 h, 48 h reperfusion following 4 h ischemia. The levels of HO-1 mRNA and protein were measured at different times by Northern blot and Western blot.

采用夹闭大鼠腹主动脉下段造成双下肢缺血和再灌注性肺损伤模型,分别采集假手术组,缺血4 h组及缺血4 h再灌注4、8、16、24、48 h组肺组织标本,以Northern印迹、Western印迹及免疫组化分析,观察HO-1表达的变化。

METHODS:Two hundred and seventy-one eyes of 219 patients with hard nucleus cataract, grading level IV or V, were operated with anteroposterior interceptive chop method for splitting the nucleus, and then with phacoemulsification. RESULTS:The hard nucleus of the 271 eyes were successfully routed. The average phaco time was 57.4 s.

对219例(271眼)级以上硬核白内障采取前后夹击劈核法,经充分水分离和水分层,吸除硬核前的皮质及软核后,乳化头退至前囊口上缘,劈核器轻压核下方,停止灌注让核上缘随前房变浅脱出囊袋口,乳化头顺势抵住核上缘,灌注加深前房后,乳化头滑入核后面,劈核器置核前面,前后夹击将核劈碎乳化吸出。

Objective To evaluate the early effect of intravesical instillation of hydroxycamptothecinfor prevention of postoperative recurrence of superficial bladder cancer.

目的 评价羟基喜树碱膀胱内灌注预防浅表性膀胱癌术后复发的近期疗效方法对36例浅表性膀胱癌患者术后应用HCPT20mg/30ml作膀胱内灌注,每周1次,共12次。

BACKGROUND: Recent studies have demonstrated that phenolic alkaloids from Menispermum dauricum can protect the heart and brain from ischemia/reperfusion injury, and promote neuron survival by inhibiting neuronal Bax and upregulating Bcl-2 expression following ische-mia/reperfusion.

背景:近来实验证明,蝙蝠葛酚性碱有保护心脑缺血再灌注损伤的作用,能通过抑制缺血再灌注后神经元bax和上调Bcl-2表达促进神经元存活。

Methods 100 cases were randomized into treatment group in which 50 cases were treated with Safflower injection via carotid artery infusion, added Safflower injection 20ml into 0.9% sodium chloride injection 60ml with the 4ml/min velocity, once a day; control group in which 50 cases were treated with venous drip of Safflower injection, added Safflower injection 20ml into 0.9% sodium chloride injection 500ml. After therapy, the curative effect、 myodynamia and hemorrheology were compared between the two groups.

将100例脑梗死患者随机分为红花注射液颈动脉灌注组50例和红花注射液静脉滴注组50例,治疗组:于0.9%氯化钠注射液60ml中加入红花注射液20ml,以4ml/min的滴速经颈动脉灌注;对照组:以红花注射液20ml加入0.9%氯化钠注射液500ml中静滴,20天后对两组疗效进行分析并观察治疗前后肢体肌力及血液流变学变化。

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