灌注的

Methods The donor rat spleen was continuously perfused with WZD to select the best protocol. Then the lymphopoiesis was examined with mixed lymphocyte culture and the splenic cells were observed microscopically and immunohistochemically.

以WZD溶液持续灌注移植脾，筛选最佳方案，并观察灌注后混合淋巴细胞培养（mixed lymphocyte culture，MLC）淋巴细胞增殖情况及脾细胞的超微结构，以及灌注后移植脾细胞的免疫组化情况。

Methods Focal cerebral ischemia and reperfusion model in male adult SD rats was established by occluding the right middle artery with 40 nylon thread blood flow blocked for 90 minutes and then reperfusion for 1 day, 7 days and 14 days.One microliter MK801 (5mmol/L and NBQX (50mmol/L) were respectively injected into the cerebral ischemic area of SD rats under stereodirectional instrument right after ischemia.

以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型(阻塞90min再灌注1d、7d和14d)，分别向其大脑皮质缺血区立体定向注射5mmol/L 的MK801和50 mmol/L的NBQX各1μl，用免疫荧光组织化学法检测脑缺血再灌注后成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧NG2和O4阳性细胞数量。

Objective: The modern medical research thought that the cerebral ischemia- reperfusion injury is an important pathophsiology mechanism that causes the various cerebral disease,and apoptosis is one of the important mechanisms of the cell death in the process of the organ and the organization ischemia-reperfution.

目的：现代医学研究认为缺血再灌注损伤是大多数急性缺血性疾病的主要病理生理过程，而细胞凋亡则是器官、组织缺血再灌注损伤过程中细胞死亡的重要机制之一，因此干预细胞凋亡，从而防治缺血再灌注损伤已经引起国内外广泛关注。

The increased ARNA produced by intrapelvic infusion of DADLE was unaffected by pretreatment of rats with the NK-1 receptor antagonist, spantide I (0.1mg/ml, 20μl/min).

另外也发现，由肾盂灌注类鸦片拮抗剂，并不会改变因肾盂内压力增加而活化的肾压力性受体时ARNA的增加；而灌注DADLE也不会改变因肾盂灌注高张食盐水(600mM NaCl)而活化肾化学性受体时ARNA的增加。

Objective To observe the clinical efficacy of treatment in acute cerebral infarction by Safflower injection via carotid artery infusion.Methods 100cases were randomized into treatment group in which50cases were treated with Safflower injection via carotid artery infusion,added Safflower injection20ml into0.9%sodium chloride injection60ml with the4ml/min velocity,once a day;control group in which50cases were treated with venous drip of Safflower injection,added Safflower injection20ml into0.9%sodium chloride injection500ml.After therapy,the curative effect、myodynamia and hemorrheology were compared between the two groups.

目的 探讨颈动脉灌注红花注射液治疗急性脑梗死的临床疗效方法将100例脑梗死患者随机分红花注射液颈动脉灌注组50例和红花注射液静脉滴注组50例，治疗组：于0.9%氯化钠注射液60ml中加入红花注射液20ml，以4ml/min的滴速经颈动脉灌注；对照组：以红花注射液20ml加入0.9%氯化钠注射液500ml中静滴，20天后对两组疗效进行分析并观察治疗后肢体肌力及血液流变学变化。

The second was study on hepatocellular apoptosis during hepatic cold preservation-reperfusion. The third was investigation on effects of rewarm ischemia on the structure and function of reperfusing donor livers as well as its relevant mechanism.

实验分三个部分：离体供肝缺血-再灌注损伤模型的建立；供肝低温保存-再灌注期间肝细胞凋亡的研究；供肝复温缺血对再灌注后供肝功能的影响及相关机理。

Objective:(1) To analyze the relationship between CT perfusion parameter and some factors affecting prognosis of gastric carcinoma by multislice CT perfusion imaging and triphase incremental scanning.

目的： 1。通过胃癌16排螺旋CT灌注成像研究，探讨胃癌灌注参数与影响胃癌预后的几个病理因素之间的相关关系，明确胃癌灌注成像的临床应用价值。

Every group rats were divided into two parts with the same rats. At last the rats were redivided ,then them were killed and their brains were excluded after one hour.4 hours or one week.Results PO2 and cerebral blood flow of E-selectin 2mg/kg group were higher than that of saline and lower than that of l0mg/kggroup(p.01).The volume of cerebral infarct and blood speed of E-selectin 2mg/kg group was smaller than that of saline and larger than that of l0mg/kg group(p.01).At the same time PCh.speeds.flows and the volume of infarct were related with the time (p.01).Conclusion E-selectin can effectively increase PC2 and rCBF after ischemia and reperfusion .reducing infarct volumes and speeds of blood at the same time . Artificial synthetic E-selectin can effectively reduce the reperfusion injury following cerebral ischemia.

结果 生理盐水组，2mg／kg组，10mg／kg组PO_2值依次提高(p＜0.01)，脑皮质血流量依次提高(p＜0.01)；脑血流速度依次减低；脑梗死体积依次减少(p＜0.01)；同时在测定的时间段里PO_2和脑组织血流量值与时间成正相关(p＜0.01)；脑梗死体积和脑组织血流速度值与时间成正相关(p＜0.01)。E一选择素在脑缺血再灌注损伤中表达的实验研究中文摘要结论E一选择素能增加脑缺血再灌注损伤的脑组织氧饱和度，脑血流量，减低脑血流速度；减少脑损伤体积；从而保护了脑缺血再灌注损伤的脑组织。

Results: The CO pretreatment group received pre-exposure to 250 ppm of CO significantly reduced the expression of CHOP in the heart compared with I-R group; however, hypoxia pretreatment group showed no such effect.

吸入低浓度的CO（250 ppm）能明显改善心肌缺血-再灌注损伤；其机制可能为外源性CO缓解缺血-再灌注激发的过度内质网应激，抑制CHOP过表达，减轻ERS相关细胞凋亡的发生，从而减轻心肌缺血-再灌注损伤。

The expression of ICAM-1 and P-selectin mRNA and protein in ischemic cerebral cortex and corpus striatum in rat was observed after 1 h of cerebral ischemia and 3,6,12,24 and 48 h of reperfusion by in situ hybridizatioin and immunohistochemistry.

脑缺血区的毛细血管内皮细胞表达ICAM-1和P—selectinmRNA发生于脑缺血／再灌注后3h，ICAM—1mRNA在脑缺血／再灌注12h达到高峰（组内比较，P〈0.01）；P—selectin mRNA高峰出现在脑缺血／再灌注6-12h（组内比较，P〈0.01）。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。