灌注液

After placement in a stereotaxic apparatus, A microdialysis probe was implanted in the left hippocampal CA1 region (2 mm to the left and 3.6 mm posterior to the bregma and 2 mm below the cortical surface).

切开头皮，用微型颅钻分别在左右海马CA1区(前囟3.6mm和中线左、右旁开3mm)打开一直径约2mm的圆孔，将微透析探针刺入左侧海马CA1区2mm，用乳酸钠林格氏液以2.5μl/min的速度持续灌注，稳定60min待脑电图波形稳定后收集脑组织透析液，间隔时间为10min，直至再灌注后2h。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Results all models were successfully established. conclusion the isolated rat lung can be used as oxygenator instead of a mechanical oxygenator in the rat cpb model.

为研究cpb中肺保护液灌注及相关药物与手段的肺保护效果，以离体肺作为cpb氧合器，建立了32例大鼠体外循环及肺保护液灌注模型，效果良好。

The effect of prevention of rat hepatocellular oedema by self-designed KYL solution are worse than that of UW solution.

自制的KYL液对大鼠肝脏的保存效果总体上与UW液相当，在再灌注后肝细胞胆汁分泌方面和钙拮抗方面略优于UW液，而在防止细胞水肿方面较UW液稍差。

The study indicated that the effects of self-designed KYL solution on rat liver preservation are similar to that of UW solution.

结果 KYL液保存的大鼠肝脏在保存16 h以内各时相胆汁流出量均较UW液保存者高（P＜0.01，灌注流出液AST、ALT和LDH含量与UW液保存者相近，肝细胞内钙离子浓度较UW液保存者低（P＜0.01； KYL组保存24及48 h时，MDA含量低于UW组，SOD含量高于UW组（P＜0.01）；光、电镜观察两者形态学变化基本一致。

ABSTRACT: Objective To observe the possibility that the proteomics of the tumor interstitial perfusing fluid can be analyzed as the tumor microenvironment proteomics.

目的 探讨肿瘤间质灌注液蛋白质组作为分析肿瘤组织中细胞微环境蛋白质组的可能性。

Methods Using modified Ferriers ischemia-reperfusion induced arrhythmic model in isolated guinea pig right ventricular wall.In this model,the preparation was superfused with modified ischemic Tyrodes solution for 10 min then reperfusioned with normal Tyrodes solution.

修改的Ferrier豚鼠离体右心室壁缺血-再灌注心律失常模型，即标本经模拟缺血台氏液灌流10min，而后用正常台氏液行再灌注。

Vitrectomy allows us to manage otherwise untreatable ocular pathologies, but the substitution of the vitreous by an artificial intraocular irrigation solution would probably disturb the physiology of the retina and choroids.

随着玻璃体切除术的出现和日益发展，眼内灌注液作为术中使用的玻璃体替代物，直接影响视网膜和脉络膜的正常生理功能。

We conclude that HES solutions, when given in doses of 20 mL/kg in connection with the CPB prime, compromise hemostasis after cardiac surgery. This effect appears related to formation of a less stable thrombus compared with that formed in the presence of ALB.

我们得出结论：使用20 mL/kg 的 HES 作为 CPB 的灌注液可损害心脏手术术后止血功能，这一效果显示其与使用 HES 相比 ALB 较少形成稳定的血栓具有相关性。

Rat orthotopic liver transplantation model was established. Perfusion and preserving solution at 4 ℃ were miltiorrhiz Ringer's lactate solution (60 mL/L) in the miltiorrhiz experimental group and Ringer's lactate solution without miltiorrhiz in the model control group.

实验方法：建立原位肝移植模型，在供肝灌注冷保存时，以4℃乳酸林格氏液为基液，丹参注射液组灌注保存液中加60 mL/L丹参注射液；模型对照组不加丹参。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。