灌注法

Methods Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

以实验性高脂血及轻度氧化LDL局部保留灌注的家兔股动脉为模型，采用DOTAP阳离子脂质体包裹LNCX-anti-MCP-1质粒，将反义MCP-1 cDNA定位导入家兔股动脉鞘及周围组织，以原位杂交、 Northern杂交及狭缝杂交法观察反义MCP-1基因的表达，应用扫描电镜观察单核细胞在动脉内膜粘附的情况。

METHODS: This I/R model was produced by bilateral carotid artery coupled with subclavia artery occlusion.

采用改良的大鼠四血管结扎法，复制大鼠急性全脑缺血（30 min）再灌注（5 min，60 min）损伤模型。

Methods ROLTs were performed according to Kamada's two cuff technique with some modifications in donor liver acquirement, perfusion, cuff made, recipient anesthesia, anastomosis of suprahepatic inferior vena cava and shorten anhepa...

材料和方法：应用Kamada&二袖套法&行大鼠肝移植，在既往国内外文献报道的基础上对供肝切取、灌注、袖套管制作、受体麻醉、肝上下腔静脉吻合和缩短无肝期等方面进行改进，熟练实验操作。

The donor liver was cold perfused through abdominal aorta and portal vein respectively, the liver of recipient was step-excised, the suprahepatic vena cavewas sutured end -to -end with 8-0 nylon line, and the continuity of infrahepatic VC and portal veinwere established by means of cuff method respectively.

作者在参考Kamada等[1，2]用&二袖套法&吻合血管的基础上，并对供肝的灌注、受体肝脏切除及移植肝脏的血流重建等进行改进，以提供一个稳定可靠的动物模型的建立方法。1材料与方法1.1实验动物、药品及术前准备采用健康、成年、雄性、清洁级体重约200￣300g SD大鼠和二级Wistar大鼠，饲养条件符合SPF标准由南方医科大学南方医院实

Methods Right middle cerebral artery sutu...

采用大鼠大脑中动脉缺血再灌注线栓法模型，缺血2h。

Objective To observe the change of gastric air tonometric pH, oxygen delivery ,oxygen consumption and gastric mucosal perfusion during cardiopulmonary bypass, during CPB.

内容摘要：作者：胡序凯吴国荣吴一霄陈骏萍目的观察体外循环中空气法胃粘膜pH值与氧供、氧耗的变化了解体外循环期间胃粘膜的血流灌注。

Methods: The experiment models of cerebral ischemia - peperfusion were made with tri - vasoligation, which 24 healthy rabbits are divided into pseudo - operative group, model group and the therapy group of Naokang capsule.

选用健康家兔24只，随机分为假手术组、造模组和脑康胶囊治疗组，采用三血管阻断法建立实验性脑缺血再灌注模型。

Result:The sterandryl substitution group and the vacation surgery group comparatively castrates the group brain stem dead volume obvious increase; The Bax protein expression masculine cell number expression also increases.

线栓法建立大鼠局灶性脑缺血再灌注损伤模型，TTC染色测脑梗死体积及免疫组化染色检测脑组织Bax的表达。结果：丙酸睾酮替代组和假手术组较去势组脑梗死体积明显增加；Bax蛋白表达阳性细胞数表达也增加。

Methods: Renal IRI models were made in rats(the right kidney was resected, and the left renal artery was closured by a clip for 1 hour and then was loosened to reperfusion). The concentration of TNF-a, IL-8 and IL-6 were detected by double antibody sandwich enzyme-linked immunosorbent assay. Using biochemical analyzer, concentration of serum urea nitrogen and creatinine were detected.

采用大鼠肾I-R模型(将大鼠右肾切除，夹闭左肾蒂1h再，松开使左肾再灌注)，用双抗体夹心ELISA法动态检测肾组织和血液中TNF-a、IL-8、IL-6含量，以及用生化分析仪测定SUN、Scr等指标变化。

WANG Wei-Ting, GUO Lian-Jun, QU Ling, WANG Fang, LU Qing, QIAN Jia-Qing(Department of Pharmacology, Tongji Medical College, Huazhong Universityof Science and Technology, Wuhan 430030, China)By using decapitating, intravenous injection of saturated MgCl2 and legation of bilateral carotid arteries with vagi, theeffects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethyl- amino) propane hydrochloride on survival time in mice were studied. With the model of cerebral ischemia for 20 min and reperfusion for 10 min, effects of DDPH on the superoxide dismutases activity and malondialdehyde content in brain tissue and pathological changes were studied.

采用断颅，iv饱和MgCl2溶液及结扎双侧颈总动脉和迷走神经等法造成小鼠脑缺血模型，观察1-(2，6-二甲基苯氧基)-2-（3，4-二甲氧基苯乙氨基）丙烷盐酸盐对小鼠脑缺血后存活时间的影响；采用小鼠脑缺血（20 min）再灌注(10 min)模型，观察DDPH对脑组织超氧化物歧化酶活性，丙二醛含量及组织病理损伤的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。