灌注法

A modification of two-step perfusion protocol and isopycnic centrifugation were used to isolate oval cells from liver. Primary oval cells were cutured in LE medium in vitro.

经门静脉灌注消化法和等密度离心法分离卵圆细胞，并在体外进行原代培养。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

Tongnaohuoluo acupuncture can increase effectively the blood supply for brain tissue cells, promote the rebuilding of collateral circulation, improve and recover the symptoms of function disorders including hemiplegia and logomania as early as possible,so it is effective in treating acute ischemic stroke.

通脑活络针刺法能有效增加大脑组织细胞血液再灌注量，加速侧支循环重建，使偏瘫、失语等功能障碍的症状尽早改善和恢复，疗效确切。

Methods: The string inserting method according to Longa＇s report was used to induce the model of rats with focal cerebral ischemia/reperfusion.

采用Longa报道的线栓法制备大鼠局灶性脑缺血-再灌注模型。

INTERVENTION: The models of ischemia/reperfusion in SD rats were established by middle cerebral artery occlusion with a nylon monofilament suture. The rats were randomly divided into the treatment groups 32cases and the control group 32 cases. Each group was divided into eight subgroups, which consisted of 4 rats at 2 hours, 6 hours, 12 hours, 24 hours, 2days , 3 days, 7 days and 14 days after reperfusion of MACO.

干预措施：成年健康雌性SD大鼠68只，应用线栓法建立SD大鼠大脑中动脉阻塞再灌注模型，随机分为治疗组32只和对照组32只，每组再随机分为缺血1.5 h再灌流2，6，12，24 h，2，3，7，14 d组(n=4)，另外4只作假手术组。

Results In vitro recovery of the probe was mainly influenced by the perfusion rate. When the perfusion rate was set at 1.0μL/min, in vitro and in vivo recoveries of the probe were 50.16±2.34% and 14.096±0.58%, respectively. The enhancement indexes for 3%, 5% mentha oil were 3.8 and 17.6, respectively. The enhancement indexes were 9.2 and 24.8, for 3% and 5% of Azone, respectively. Conclusion The steady sate flux of meloxicam in rats is significantly improved by enhancer mentha oil and azone.

结果： 在实验条件下微渗析探针的体外回收率主要受灌注液流速的影响，为（50.16±2.34）%；反向渗析法测得探针的在体回收率为（14.096±0.58）%；MO的体积分数为3%和5%时，增渗倍数分别为3.8，17.6；azone的体积分数为3%和5%，增渗倍数分别为9.2，24.8；结论：不同体积分数的MO和azone均可显著增加美洛昔康经皮渗透稳态流量，azone的体积分数为5%时，大鼠角质层的屏障作用可能受到了较大程度的破坏。

Methods: R-Y limb motility of 13 cases was measur ed with perfused manometry system and R-Y limb mucose was biopsied t o perform bacterial culture.

采用多孔导管灌注测压法，测定13例术后病人R-Y空肠袢动力变化并作其粘膜细菌学检查。

objectiveto investigate the effect of myricetin dispersible tablets on experimental cerebral ischemia-reperfusion injury in rats and thrombosis formation.methodsexperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. the protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, sod and mao activities,mda and la contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型，通过测定大鼠脑含水量，超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量，观察杨梅素分散片对大鼠实验性脑缺血的影响；采用体内静脉血栓形成法，观察杨梅素分散片对体内静脉血栓形成的影响；并研究其对血小板聚集的影响。

MethodsExperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. The protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, SOD and MAO activities,MDA and LA contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型，通过测定大鼠脑含水量，超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量，观察杨梅素分散片对大鼠实验性脑缺血的影响；采用体内静脉血栓形成法，观察杨梅素分散片对体内静脉血栓形成的影响；并研究其对血小板聚集的影响。

Underwater concrete of bored piles were poured by lifting conduit method, while overwater concrete pouring adopted drawing ball technique.

钻孔灌注桩水下混凝土采用提升导管法施工，水上混凝土灌注采用拔球的施工技术，介绍了施工注意事项及应对措施。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。