灌注法

Methods A pathological model of cerebral ischemia reperfusion damage in rats without craniectomy was developed referring to Koizumi and Longa's method.

参照Koizumi和Longa's法建立大鼠不开颅的脑缺血一再灌注损伤病理模型。

The ethological index of gerbils was counted by the open field method after reperfusion.

用开阔法观察沙土鼠脑缺血再灌注后行为学改变。

As for the animalmodel, we duplicated MCAO/reperfusion model on SD rats via reformed nylonmonofilament method and did obsevation and evaluation on early ethological andhistological changes, the constancy and reproducibility are satisfactory.

在模型方面我们采用了与临床病人发病情况相类似的SD大鼠MCAO局灶性脑缺血再灌注模型，运用早期行为学及组织学观察评价，通过改良线栓法成功复制了此模型，稳定性、重复性较好。

Manganese oxides supported on SBA-15 Mn(subscript xO/SBA-15 were prepared by incipient wetness impregnation of SBA-15 with furfuryl alcohol solution of manganese acetylacetonate or manganese nitrate followed by removal of the carbon resulted from carbonized FA.

采用溶液浸渍法将乙酰丙酮锰或硝酸锰的糠醇溶液分别灌注到SBA-15的孔道内，一定条件下将糠醇碳化后再将碳烧除，即可得到高度分散的MnO/SBA-15复合催化剂。

Methods: To reproduct the mouse model of vascular dementia through operation of cerebral ischemia reperfusion, and contrast to Naofukang. Then, to detect the praxiological changes of each group mouse by the means of water maze and the activity of SOD and the content of MDA in hippocamp on the 14th day after operation.

来用脑缺血再灌注的方法复制血管性痴呆小鼠模型，以脑复康为对照，通过水迷宫法分别观测术后14天各组小鼠行为学以及脑海马SOD活性、MDA含量的变化。

Methods to reproduct the mouse model of vascular dementia through operation of cerebral ischemia reperfusion, and contrast to naofukang. then, to detect the praxiological changes of each group mouse by the means of water maze and the content of cgrpand ss in hippocamp on the 14th day after operation.

方法采用脑缺血再灌注的方法复制血管性痴呆小鼠模型，以脑复康为对照，通过水迷宫法分别观测术后14天各组小鼠行为学以及脑海马cgrp和ss含量的变化。

The Sham rats were only performed the incision on cervix but without any treatment on the arteries. Apoptosis neurons in hippocamp CA1 subregion were observed at 72 hours after global brain ischemia.

在脑缺血再灌注后72小时，杀死大鼠，取海马组织依POD法作凋亡细胞检测，计数各组大鼠海马CAI区凋亡细胞数。

The spinal cord ischemia-reperfusion model was made by clamping the infrarenal aortic in rabbits.

采用肾下腹主动脉阻断法，建立脊髓缺血再灌注损伤模型。

Methods Wistar rats were randomly divided into toxicosis groups and control group by intratracheal instillation.

采用气管直接注入染毒法，分别给大鼠灌注0，1.5，7.5，37.5mg/kg剂量的颗粒物悬浮液。

A modification of a twostep perfusion protocol and isopycnic centrifugation were used to isolate oval cells from livers.

经门静脉灌注消化法和等密度离心法分离卵圆细胞，并在体外进行长期培养。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。