灌注法

Methods: In different time, the concentrations of CINC and the activities of myeloperioxidase were observed in experimental cerebral isc hemia reperfusion model with Sprague-Dawley rats made by modified thread em bolism method.

选用改良线栓法复制Sprague-Dawley大鼠脑缺血再灌注实验模型。对不同时间内的血清MPO活性及CINC水平进行测定。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末，再灌注1、2、4、8、12h，测定各组血浆中的MDA、骨骼肌组织中的MPO，血清嗜中性粒细胞化学趋化因子(CINC／IL-8)，血清一氧化氮，用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b／CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

V/Q in one day procedure is feasible and sensitive for the diagnosis of pulmonary embolism. V/Q and spiral CT are complemental.

一日法V/Q对PE有很好的诊断价值，肺通气显像提高了肺灌注显像诊断的特异性。V/Q与螺旋CT对PE的诊断两者为互补关系。

METHODS Expression of Bcl-2,Bax and P53in endothelial cells of cremaster ischemia-reperfusion of the rat was investi-gated by means of immunohistochemical SP method.

以免疫组织化学SP法对大白鼠提睾肌缺血再灌注后血管内皮细胞Bcl-2，Bax及p53的表达进行观察。

Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.results it was found that hpk1 was expressed in rat hippocampus neurons.conclusions administration of hpk1 antisense oligodeoxynucleotides can significantly protect hippocampus neurons from apoptosis without dose-dependence.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。结果在大鼠海马神经元中有hpk1的表达，脑室注射as-odns大鼠海马ca1区存活锥体细胞明显增多。结论 hpk1反义寡核苷酸对缺血/再灌注引起的海马神经元凋亡具有保护作用。

With practical examples, crosshole sonic logging is employed in this paper to inspect the integrity of the cast-in-place pile and to determine the degree of defectiveness.

用实例介绍声波透射法在混凝土灌注桩桩身完整性检测中的应用效果，确定缺陷的范围。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

Methods Forebrain ischemia model of gerbil was produced by occlusion of bilateral carotid arteries for 5 minutes.

采用双侧颈总动脉阻断法制备沙土鼠（64只）前脑缺血再灌注损伤模型，缺血5分钟。

ABSTRACT Objective: To investigate the effects of low level laser irradiation on acupuncture points combined with iontophoresis against brain damage after middle cerebral artery occlusion in rats.

摘要］ 目的：观察激光透穴离子导入法对大鼠大脑中动脉缺血再灌注模型损伤的影响。

Methods Roux-Y limb motility of 13 cases was measured with perfused manometry system.

采用多孔导管灌注测压法，测定13例全胃切除术后病人Roux-Y空肠袢动力变化。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。