灌注法

Methods One hundred patients with heart disease were divided into two groups:Group of pulsatile perfusion and group of continuous nonpulsatile flow perfusion.

将100例心内直视手术患者，按灌注方法不同分为搏动灌注组与对照组，每组各50例，分别采用搏动灌注法与平流灌注法。

Of chronic salpingitis in traditional Chinese and western medicine combined treatment with surgery better:(1) anti-inflammatory treatment: antibiotics and adrenal cortical hormone treatment (2) drug bureaucratic perfusion: Infertility of tubal blockage, the infusion of drugs to promote inflammation absorption.

对慢性输卵管炎以中西医结合配合手术综合治疗效果较好：(1)抗炎治疗：用抗生素与肾上腺皮质激素联合治疗(2)药物官腔灌注法：对输卵管堵塞不孕者，灌注药物促进炎症吸收。

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞，接种并预培养 1 。5 h后，用含不同浓度 HGF的培养基继续培养至 48h，MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

In 66 anaesthetized rats, the effects of local injection of agmatine on femoral, renal, and mesenteric vascular beds were investigated respectively by constant flow perfusion method.

在66只麻醉大鼠，分别采用后肢、肾脏和肠系膜动脉在体恒流灌注法，观察了向灌流环路中直接注射胍丁胺(agmatine， AGM)的血管效应，以所引起的灌流压增减反映血管的收缩和舒张。

The results of this study suggested that different activated sludges could tolerate the concentration of 25% NaCl by the acclimatized procedure and decolorize the simulated dye wastewater with high salt concentration in anaerobic conditions.

食品、纺织、染料、制药和化工/石油等行业，在生产过程中产生大量的含盐废水，含盐量有时高达15%~20%[2，3]。目前，对此类废水一般采用电解法、膜分离法、焚烧法和深井灌注法等技术处理。

Methods: The human endothelial cells of umbilical vein and rat microvascular endothelial cells were obtained by methods of perfusion/digestion and histiocytic attachment using typeⅠcollagenase (0.1%). The morphologic characteristics of endothelial cells were observed under inverted microscope.

采用0.1%Ⅰ型胶原酶消化灌注法和组织块贴壁法分别获得人脐静脉内皮细胞和大鼠微血管内皮细胞，每天在倒置相差显微镜下进行观察，并用免疫荧光法分别进行鉴定。

Methods: Rat infrarenal AAA models were created via infusion . Porcine pancreatic elastase was infused in treated group and saline was infused in control group .The change of NF-κB was detected by immunohistochemistry and was analyzed by computer image analysis.

采用灌注法制备大鼠AAA模型，实验组采用猪胰弹力酶灌注，对照组采用生理盐水灌注，免疫组化法观察NF-κB的阳性变化，计算机图像分析仪分析其变化规律。

Methods:The artificial defects of 20 rabbits were repaired with frozen allogeneic mandible and fresh syngeneic mandible to study the revascularization's dynamic process of mandible transplantation by the inkgelation vascular perfusion,vascular corroisioncast,Nuclein scintigraphy;the 85 cases received frozen allogeneic mandible transplantation and the measures to improve revascularization;The defects were implanted with bFGF and allogeneic bone,the defects were only repaired with allogeneic bone as control group.

通过冷冻异体下颌骨和新鲜自体骨移植修复20例家兔下颌骨缺损的动物实验，经墨汁明胶血管灌注法、血管铸型法、放射性核素骨显像研究了异体下颌骨移植再血管化及骨改建的动态过程；同时另20例采用bFGF和异体骨修复作为实验组，单纯异体骨修复作为对照组，植入后1个月、3个月行墨汁明胶血管灌注透明法及组织学观察。

Methodsthe Ginger oil were loaded on the slides, and the time of killing the Schistosoma Cercariae was recorded.

取生姜油100μl于载玻片上，观察生姜油杀灭血吸虫尾蚴的时间；并用已杀灭的血吸虫尾蚴感染小鼠，经灌注法加撕碎法收集虫体，计算感染率。

Methods: Hepatic stellate cells were derived from normal rats by in situ perfusion, digestion and density gradient centrifugation with 108 g/L Nycodenz. The cells were stimulated by various concentrations of PDGF or MDA, and then treated with SAB.

采用原位灌注法消化大鼠肝脏，108g/L Nycodenz密度梯度离心，分离HSC，以MTT法观察细胞的增殖能力，免疫组化法检测血小板生长因子受体含量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。