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A modification of a twostep perfusion protocol and isopycnic centrifugation were used to isolate oval cells from livers.

经门静脉灌注消化法和等密度离心法分离卵圆细胞,并在体外进行长期培养。

A modification of two-step perfusion protocol and isopycnic centrifugation were used to isolate oval cells from liver. Primary oval cells were cutured in LE medium in vitro.

经门静脉灌注消化法和等密度离心法分离卵圆细胞,并在体外进行原代培养。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC;用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC;采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

During intestinal ischemia/reperfusion in mice, anti-SDF-1 and anti-CXCR4, but not isotype control antibodies, abolish the recruitment of CD34 cells in microcirculation.

在小鼠小肠缺血再灌注实验中,非同型调节抗体抗SDF-1和抗CXCR4,阻断了CD34 细胞在微循环的募集。

During intestinal ischemia/reperfusion in mice, anti-SDF-1 and anti-CXCR4, but not isotype control antibodies, abolish the recruitment of CD34+ cells in microcirculation.

在肠缺血/再灌注小鼠模型中,抗-SDF 1、抗-CXCR4减弱CD34+细胞在微循环中的募集,但对照抗体的异构体却无此作用。

We investigated the jejunal mucosal capacity to autoregulateperfusion during nonpulsatile CPB (34° C) in 10 patientsundergoing elective cardiac surgery.

我们研究了10例行择期心脏手术的患者非搏动血流 C PB (34° C)期间空肠粘膜对灌注的自身调节功能。

Objective To investigate the effect of jejunal casein perfusion on pancreatic exocrine secretion in experimental acute necrotic pancreatitis rats and analyze the neuromechanism that may be involved.

目的 观察空肠内灌注酪蛋白对急性坏死性胰腺炎大鼠胰腺外分泌的影响,并初步探讨其神经机制。

The effect of prevention of rat hepatocellular oedema by self-designed KYL solution are worse than that of UW solution.

自制的KYL液对大鼠肝脏的保存效果总体上与UW液相当,在再灌注后肝细胞胆汁分泌方面和钙拮抗方面略优于UW液,而在防止细胞水肿方面较UW液稍差。

The study indicated that the effects of self-designed KYL solution on rat liver preservation are similar to that of UW solution.

结果 KYL液保存的大鼠肝脏在保存16 h以内各时相胆汁流出量均较UW液保存者高(P<0.01,灌注流出液AST、ALT和LDH含量与UW液保存者相近,肝细胞内钙离子浓度较UW液保存者低(P<0.01; KYL组保存24及48 h时,MDA含量低于UW组,SOD含量高于UW组(P<0.01);光、电镜观察两者形态学变化基本一致。

Compare with the ability of eliminate the foam, Throught the test to discover, the taper of the diffuse pipeline of the eliminating-foam equipment can not small, the taper of the diffuse pipeline greater than 79/350; The length of the diffuse pipeline of the eliminating-foam equipment greater than 350mm; Whether the speed of the foam can too high, the velocity of the foam 0.3~0.6m/s; The width of lacune is between 0.2 and 0.5mm.

由于泡沫的流变性难以控制和测试,其变化较大,提出以利用所有泡沫钻探生产的钻进压力值,利用数学分析的方法对这些钻进压力值进行分析,在此基础上总结出更加合适、简捷的泡沫灌注压力公式。

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