灌注

Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化；并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组（n=10）及葛根素组（n=10），所有动物均阻闭腹主动脉肾下段20min，建立兔脊髓腰尾段缺血模型。

Methods A pathological model of cerebral ischemia reperfusion damage in rats without craniectomy was developed referring to Koizumi and Longa's method.

参照Koizumi和Longa's法建立大鼠不开颅的脑缺血一再灌注损伤病理模型。

Reversible middle cerebral artery occlusion without craniectomy in rats［J］.

红景天苷对大鼠局灶性脑缺血再灌注损伤的保护作用［J］。

In this work, with the bilateral carotid artery stroke model in Mongolian gerbils, the production and changes of NO and ROS in the pathogenic process of stroke were investigated, and the protective effects and mechanism of Crataegus extracts , a kind of natural antioxidant, on the ischemia/reperfusion damage were also studied.

本工作以蒙古沙鼠双侧颈动脉结扎引起的脑损伤中风模型，研究了中风病程中活性氧和一氧化氮的产生变化，同时探索了一种天然抗氧化剂—山楂提取物(Crataegus Extracts，CE)对缺血再灌注脑损伤的保护作用及机制。

The remaining 25% of causes can be attributable to systemic hypoperfusion, hypercoagulable states, and cryptogenic causes.

1其余25 ％的原因可以归因于血流灌注不足，高凝状态，或者其他不明的原因。

METHODS: The expression of Bax was detected by flow cytometer in cortical neurons under ischemic like conditions.

利用流式细胞仪对小鼠皮质神经元类缺血再灌注损伤后Bax的表达进行检测。

METHODS: The expression of Bax was detected by flow cytometer in cortical neurons under ischemiclike conditions.

利用流式细胞仪对小鼠皮质神经元类缺血再灌注损伤后Bax的表达进行检测。

Cytophaga sp was selected for heparinase production because it was more productive. Three heparinases, named HⅠ, HⅡ and H Ⅲ were purified from the crude cell extract by means of batch DEAE-Cellulose negative adsorption, Hydroxylaptide adsorption and releasing, SP-Sepharose cation exchange chromatography, Source 30S cation exchange chromatography, Poros 20HS cation exchange perfusion chromatography and Sephacryl S200HR gel permeation chromatography.

利用超声破碎、DEAE-纤维素反吸附去杂、羟基磷灰石批处理吸附层析、SP-Sepharose离子交换层析、Source 30S离子交换层析、Porous20HS离子交换灌注层析及Sephacryl S200HR凝胶排阻层析，从噬纤维菌中纯化出三种新的肝素酶。

Objective To study the cytotoxic effect of thiotepa and mitomycin C on human bladder cancer cell line in vitro.

目的 探讨体外膀胱灌注噻替哌和丝裂霉素C药物浓度和作用时间对膀胱癌细胞杀伤作用的影响。

Take out of the locking intramedullary pin from the patient with femur＇ s disunion,and debride thoroughly,then transplant the fibula of the patient himself to the femur＇ s medullary cavity and transplant the ilium to the location of the disunion.

对15例股骨骨折带锁髓内针内固定术后骨不连患者行取出髓内针，彻底清创，自体腓骨髓内移植，骨不连处植髂骨，全身抗感染和局部持续灌注冲洗引流。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。