灌注

Methods Ten rats were subjected to anatomy following caoutchouc perfusion into the artery by inserting tubes through the heart, 10 were subjected to dissection following artery and vein perfusion, and 10 were subjected to dissection immediately following anesthesia.

采用经心脏插管天然橡胶灌注方法对10只大鼠行动脉灌注后解剖、10只大鼠行动静脉同时灌注后解剖， 10只活体大鼠麻醉后直接解剖，观察大鼠尾动脉情况并测量尾动脉不同平面的外径。

Methods Ten rats were subjected to anatomy following caoutchouc perfusion into the artery by inserting tubes through the heart, 10 were subjected to dissection following artery and vein perfusion, and 10 were subjected to dissection immediately following anesthesia. Diameter of the caudal artery was measured.

采用经心脏插管天然橡胶灌注方法对10只大鼠行动脉灌注后解剖、10只大鼠行动静脉同时灌注后解剖， 10只活体大鼠麻醉后直接解剖，观察大鼠尾动脉情况并测量尾动脉不同平面的外径。

Objectives: To observe the influence of Danshen to the expressions of mRNA and protein of local hypoxia inducible factor-1α(HIF-1α) during acute ischemia reperfusion injury of skeletal muscles in SD rats, and its effects on the changes in erythrocyte aggregation index, erythrocyte deformation index, local swelling coefficient and histology of the left cremaster muscles of SD rats during ischemia reperfusion injury, therefore to discuss the influence of Danshen to the oxygen environment and hemorheology of skeletal muscle during ischemia reperfushion injury.

目的：通过建立SD大鼠左侧提睾肌的缺血再灌注损伤模型，观察丹参对SD大鼠骨骼肌缺血再灌注损伤局部缺氧诱导因子1α（Hypoxia Inducible Factor-1α，HIF－1α）mRNA及其蛋白表达的影响、对红细胞聚集和变形指数的变化、局部肿胀系数的变化以及组织形态学改变的作用，探讨丹参对缺血再灌注损伤骨骼肌局部氧环境及血液流变性的影响。

RESULTS: After ischemia-reperfusion injury, the rats had hydrouria, urine osmotic pressure depress, symptoms of carnine and urea nitrogen increasing. HE staining demonstrated that renal tubular epithelial cells were swelling, necrosis, and desquamate. Aquaporin-1 expression and its mRNA level was decreased; in particular, the expression and level were the lowest at day 1 after ischemia-reperfusion injury and recovered to normal value at day 5 after ischemia-reperfusion injury.

结果：大鼠肾脏缺血再灌注损伤后出现尿量增多、尿渗透压降低，血肌苷、尿素氮升高症状；苏木精-伊红染色示肾小管上皮细胞肿胀、坏死、脱落；水通道蛋白表达量及其mRNA含量均较对照组减少，这些变化于缺血再灌注损伤 1 d时最低，至缺血再灌注损伤 5 d时恢复正常。

Methods The donor rat spleen was continuously perfused with WZD to select the best protocol. Then the lymphopoiesis was examined with mixed lymphocyte culture and the splenic cells were observed microscopically and immunohistochemically.

以WZD溶液持续灌注移植脾，筛选最佳方案，并观察灌注后混合淋巴细胞培养（mixed lymphocyte culture，MLC）淋巴细胞增殖情况及脾细胞的超微结构，以及灌注后移植脾细胞的免疫组化情况。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Methods By radioimmunoassay,we measured the contents of CGRP in mesencephalon and pituitary after reperfused 5 minute following 5 minute ischemia; reperfused 45 minute following 15 minute ischemia; reperfused 30 minute following 30 minute ischemia;and compared with controls(pseudo-operation, same time of ischemia and the same total time but only ischemia).

采用放射免疫测定方法，测定大鼠全脑缺血5 min再灌注5 min、缺血15 min再灌注45 min、缺血30 min再灌注30 min中脑及垂体CGRP含量变化，并与相同缺血时间组、假手术组及总时间相同但仅有缺血组相比。

Methods Focal cerebral ischemia and reperfusion model in male adult SD rats was established by occluding the right middle artery with 40 nylon thread blood flow blocked for 90 minutes and then reperfusion for 1 day, 7 days and 14 days.One microliter MK801 (5mmol/L and NBQX (50mmol/L) were respectively injected into the cerebral ischemic area of SD rats under stereodirectional instrument right after ischemia.

以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型(阻塞90min再灌注1d、7d和14d)，分别向其大脑皮质缺血区立体定向注射5mmol/L 的MK801和50 mmol/L的NBQX各1μl，用免疫荧光组织化学法检测脑缺血再灌注后成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧NG2和O4阳性细胞数量。

Objective: The modern medical research thought that the cerebral ischemia- reperfusion injury is an important pathophsiology mechanism that causes the various cerebral disease,and apoptosis is one of the important mechanisms of the cell death in the process of the organ and the organization ischemia-reperfution.

目的：现代医学研究认为缺血再灌注损伤是大多数急性缺血性疾病的主要病理生理过程，而细胞凋亡则是器官、组织缺血再灌注损伤过程中细胞死亡的重要机制之一，因此干预细胞凋亡，从而防治缺血再灌注损伤已经引起国内外广泛关注。

Methods: Myocardial ischemia-reperfusion injury model was established through accluding the left anterior descending branch of coronary artery for 60 min and removing the ligation tater to reperfuse for 30 min.

结扎大鼠冠状动脉左前降支60min，再灌注90min复制大鼠心肌缺血再灌注损伤模型，观察心安灵对大鼠心肌缺血再灌注损伤的保护作用。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了