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Results: In groupⅢ, the expression of MDA, MPO, CINC/IL-8, CD11b/CD18, ICAM-1 were increased evidently than group Ⅰ and group Ⅱ.

结果:缺血再灌注组再灌注后各时相点MDA、MPO、CINC/IL-8以及CD11b/CD18、ICAM-1的表达较假手术组和缺血组均有非常显著升高(p.01),NO在缺血期末和再灌注早期(1h)显著升高,之后逐渐下降,至12h仍高于正常,骨骼肌组织损伤也随再灌注时间的延长而逐渐加重,SOD组及MSODa组则可以明显抑制这种变化,减轻骨骼肌损伤。

Results:In ischemia group, the peaks of MPO activity and CINC conentr ation were 24 hour and 6 hour respectively after ischemia, which initially incre ased at 1 hour, In ischemia reperfusion group, the peaks of MPO activity and CIN C conentration were 72 hour and 6 hour respectively after ischemia reperfusion, which initially increased at 6 hour and 1hour down to normal at 24hour respectiv ely.

结果:单纯缺血组MPO活性增高始于缺血6h并于缺血72h时达高峰,为0.15±0.01U/ml; CINC水平增高始于缺血1h,缺血6h时达高峰,为0.05±0.05ng/ml,以后逐渐下降并于缺血48h后恢复正常。缺血1小时再灌注组MPO活性增高始于再灌注1h并于再灌注24h时达高峰,为0.42 ±0.12U/ml;CINC水平增高始于再灌注1h,再灌注3h时达高峰,为0.65±0.03ng/ml,以后逐渐下降并于再灌注24h后恢复正常。

Methods One hundred patients with heart disease were divided into two groups:Group of pulsatile perfusion and group of continuous nonpulsatile flow perfusion.

将100例心内直视手术患者,按灌注方法不同分为搏动灌注组与对照组,每组各50例,分别采用搏动灌注法与平流灌注法。

The quality of the allograft, which undewent reperfusion during cold preservation gained an obvious advantage to the routine ones. At the same time, the quantity of the reperfusion liquid should be controlled; 10 ml reperfusion liquid can effectively lustrate the blood and the air that resided in the liver.

通过改进技术改善肝功能,提高了生存率,尤其在冷保存时经门静脉再灌注,在供肝的质量上明显优于不进行灌注组,并应严格掌握灌注液体量,10 ml灌注量明显优于其他液体量,能有效地驱除供肝内残留的血液和气泡。

At the same time, the quantity of the reperfusion liquid should be controlled; 10 ml reperfusion liquid can effectively lustrate the blood and the air that resided in the liver.Key words liver transplantation; rats; reperfusion; animal model 建立稳定大鼠原位肝移植rat orthotopic livertransplantation,ROLT

通过改进技术改善肝功能,提高了生存率,尤其在冷保存时经门静脉再灌注,在供肝的质量上明显优于不进行灌注组,并应严格掌握灌注液体量,10 ml灌注量明显优于其他液体量,能有效地驱除供肝内残留的血液和气泡。

Forty adult female rats were divided randomly into 4 groups:① Pseudo-operation control group: no ovariotomy, no common carotid artery occlusion;② Cerebral ischemia reperfusion group: with CCAO only, no OE;③ Ovariotomized control group: with OE and CCAO, no estradiol;④ Estradiol supplement group: with OE, CCAO and estradiol supplemented (30 d after ovariotomy, estradiol was supplemented every day for 14 d). All the rats were sacrificed after 12h of reperfusion injury, 10% of brain tissue was homogenized and 10% alue was determined by UV spectrophotometer, NO content was detected, and frozen sections and NOSmRNA in-situ hybridization were performed.

取SD大鼠40只随机分为4组:①假手术对照组:不切除卵巢,不夹闭颈总动脉;②脑缺血再灌注组:作局灶性脑缺血再灌注手术,不切除卵巢;③去卵巢对照组:切除卵巢,作局灶性脑缺血再灌注手术,不补充雌激素;④补充雌激素组:切除卵巢,作局灶性脑缺血再灌注手术,补充雌激素(切除双侧卵巢30d开始补充雌激素,连续2周)。

Methods Using HIRI model in tabbits,animals were randomly divided into four groups (n=10 in each),HIR group,HIR+ LGT group,HIR+ Pro group and HIR+ LGT+ Pro group,the contents of adenosine triphosphate,adenosine diphosphate,adenosine monophosphate,total adenylic acid numberand energy charge in the liver tissue were measured at 45 minutes after reperfusion, respectively.

复制肝缺血-再灌注损伤模型,随机将40只实验兔分为肝缺血-再灌注组、肝缺血-再灌注+LGT治疗组、肝缺血-再灌注+PRO治疗组和肝缺血-再灌注+LGT+PRO治疗组。在再灌注45 min时,分别观察肝组织内三磷酸腺苷、二磷酸腺苷、一磷酸腺苷含量、总腺苷酸量、能荷及肝细胞形态学的变化。

Results: Compared with IR group , XSOL could significantly decrease the cerebral water content, the cerebral index, the brain vascular permeability in rats of acute incomplete brain ischemia-reperfusion (P.01,P.05). Cerebral tissue morphology showed that neuronic damage of rats subjected to acute incomplete brain ischemia-reperfusion was ameliorated following administration of XSOL (2.4, 9.6g/kg). Compared with IR group , XSOL could significantly decrease the content of MDA and NO in brain tissue(P.01,P.05)and increase SOD,GSH-px activity. The levels of cerebral Glu,Asp were cut down(P.01,P.05). Conclusion: XSOL shows protective effects on cerebral ischemia-reperfusion.

结果:与缺血再灌注组比较,XSOL大、小剂量组可以显著降低急性不完全性脑缺血再灌注模型大鼠的脑含水量(P.01,P.05)、脑指数(P.01,P.05)、脑毛细血管通透性(P.01,P.05);光镜下脑组织形态学显示各剂量组可不同程度的减轻急性不完全性脑缺血再灌注大鼠脑组织的神经元受损形态;与缺血再灌注组比较,XSOL大、小剂量组可以显著降低缺血再灌注大鼠脑组织中的MDA的含量(P.01,P.05),升高SOD、GSH-Px在脑组织中的活性(P.01,P.05);XSOL大、小剂量组可显著降低脑组织中NO的含量(P.01,P.05),降低脑组织中Glu、Asp含量(P.01,P.05)。

Results The expression of MMP-9 was low in procerebrum at different phases in sham operated group.

结果假手术组各个时间段大鼠前脑组织少量表达MMP-9;缺血再灌注6h组大鼠脑组织即有MMP-9的表达,缺血再灌注24h组、48h组大鼠脑组织大量表达MMP-9;缺血再灌注3d、5d组大鼠脑组织亦可见MMP-9的表达,但较缺血再灌注24h、48h组相比MMP-9表达减少;除川芎嗪6h组不具统计学意义外,其它时间段川芎嗪治疗组与缺血再灌注组相比MMP-9含量均降低。

In NF animals, the food intake was significantly lower than control animals in the period of 3h and 6h after infusion of sodium propionate, in the 3h infused with low concentration sodium acetate (1M), and in all of stages infused with high concentration sodium acetate (2M).

灌注VFA盐后血浆胰岛素水平均显著性升高,丙酸钠灌注组升高的程度又显著性高于乙酸钠灌注组,在3小时时,丙酸钠灌注组胰岛素水平迅速下降到灌注前的水平,而乙酸钠灌注组胰岛素的水平却仍然保持在高于对照组的水平,但统计不显著。

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