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MethodsNeural tubes isolated from the region between midotic placode and third somite of mouse embryo at ED 8.5d were cultured in DMEM/F12 serum-free medium containing basic fibroblast growth factor and epidermal growth factor. The cell cycle of CNCCs was analyzed with flow cytometry, the proliferation capability was determined by MTT method, AP-2α expression was detected by immunocytochemistry as a biological marker of cardiac neural crest cells. The features of migration, multi-directional differentiation were observed as well.

材料与方法成年2月龄C57/BL6小鼠交配,取ED8.5d小鼠胚胎枕中部至第3体节神经管,应用包含碱性纤维生长因子和表皮细胞生长因子的无血清培养基培养,利用MTT法检测细胞增殖能力,利用流式细胞仪检测细胞周期,采用转录激活因子2α(AP-2α,Activator protein-2α)作为其生物学标记物,观察其增殖状况、细胞周期、迁移、分化等生物学特性。

Often, one or two natural compounds activated a couple of sites -- a sort of partial key.

通常有一两种天然化合物激活一两个点——某种局部的钥匙。

In the nonphysical, spearmint helps align communication by triggering a common language that all understand.

在非物质层上,绿薄荷通过激活一种万物均理解的共同语言而帮助安排交流。

Both mitochondrial Ca2+ channels are unaffected by blockers of other possibly Ca2+-conducting mitochondrial pores but were activated by spermine (1 mmol/L).

其他可能参与Ca2+转运的线粒体孔阻断剂对这2种钙通道没有作用,精胺(1 mmol/L)则可以激活二者。

Consequently, we do not use voice activation for tasks that demand split-second decisions.

因此,我们不将语音激活用于需要争分夺秒做出决定的任务。

When activated, the C/LKON signal is a square wave of approximately 163-ns period.

当被激活时,C / LKON信号是一个约有163 ns的方波周期。

We examined actiation of mTOR-signalling in relation to insulin resistance and hepatic steatosis in mice.

我们在小鼠检验了mTOR信号传导的激活与胰岛素抵抗和肝脏脂肪变性。

The steepness of the activation function.

在陡峭的激活作用。

Multiple strobe signals can be temporarily activated in an offset or staggered arrangement to reduce the frequency of the strobe signals.

多个选通脉冲信号能够以一个偏移或者交错方案被临时激活以减小选通脉冲信号的频率。

The changes of signal intensity were synchonous. However, there was no significant change in other subcortex regions.

上述4个区域信号的抑制变化过程同步;皮层下其他区域包括海马基底节区未引出激活点。

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The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性,因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊,这是第二次你打败了我!

So a kind of strong antagonism permeated in the relations of two countries.

所以两国这一时期的政治关系始终弥漫着一种强烈的对抗情绪。