激活

The present study was conducted to investigate the effect of different culture systems, co-culture cells and their sequence culture on the in vitro development of porcine parthenogenetic embryos derived from in-vitro matured oocytes.

经体外成熟、孤雌激活和培养获得猪胚胎，研究了不同培养体系、共培养体细胞和序贯培养对猪孤雌激活胚胎发育的影响。

AIM: To investigate the effects of T cell activators on the mRNA expression of recombinationactivating genes in Jurkat cells.

目的：研究T细胞激活剂PHA，抗CD3 mAb， PMA+A23187对人T细胞白血病细胞株Jurkat重组激活基因mRNA表达水平的影响。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberines function may be downregulating the expression of ICAM-1 on the HUVEC .

结果：小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附，其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达；小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附，对淋巴细胞表面粘附分子CD18的表达无影响。

In this study, we could not find significantly less root resorption lacuna and significantly less TRAP activity in the IL-1 group than in normal group, but there was significant difference betweens TNFα group and normal group.

其中IL-1调控于破骨细胞前体的分化阶段，TNF α调控成熟破骨细胞功能激活阶段。2：RANKL／RANK调控系统既作用于破骨细胞前体的分化、成熟，又作用于功能激活阶段。

It is now generally accepted that interactions between ligand-receptor complexes and cofactors are essential for AR function, selectivity, sensitivity, and may contribute to the progression of prostate cancer.

我们应用PKC激动剂PMA对AR进行的研究表明，PMA对野生型AR和AR突变体均有基本相同的配体依赖性转录激活作用，并且还与DHT一起，对野生型AR及AR突变体的转录激活起协同的作用。

Cultured PC12 cells were incubated with Fluo 3-AM working solution (the final concentration of Fluo 3-AM is 5μmol/L) at 37℃for 45min, followed by 3 times washes with the culture medium. The cultured neurons grown on glass coverslips were placed in a cell chamber on the stage of an inverted microscope.

多种应激刺激通过细胞内信号转导途径使CREB第133位的丝氨酸磷酸化而激活，活化的CREB直接或间接激活相关基因的转录，进而表述某些蛋白分子以及某些神经营养因子等，进而调节神经元在应激性损伤后再生、存活及修复等。

Activation of the surface DORs causes a slow Ca〓 elevation by both inositol 1, 4, 5-trisphosphate receptor-mediated intracellular Ca〓 release and extracellular Ca〓 entry, resulting in a slow and long-lasting increase in intracellular Ca〓, exocytosis, DOR insertion and CGRP release.

激活DOR引起的DOR的上膜是依赖于LDCV与细胞膜的融合和细胞内的钙离子升高。激活DORs可以引起胞内IP3钙库的释放，和一种不通过电压依赖性钙通道的外钙内流；从而引起DOR的上膜和神经肽的分泌。

The effect of AA on BEL-7402 was not significant; However, AA could effectively enhance AT-induced hepatocarcinoma cell apoptosis and lesion severity through activation of caspase-3 but not ERKs-Conclusion; Caspase-3 and ERKs proteins could involve in arsenic-induced hepatocarcinoma cell apoptosis and differentiation respectively as intracellular signaling molecules; The effect between AT and AA on hepatocarcinoma is synergistic, which further inhibits cell growth and induces apoptosis in human hepatocarcinoma cells through activation of caspase-3 but not ERRs.

AA对BEL-7402无明显的作用，但能有效的通过caspase-3而不是ERKs的激活促进AT对肝癌细胞的凋亡效应。结论：ERKs和caspse-3信号蛋白可能分别参与了砷剂诱导的人肝癌细胞促分化和凋亡效应，AT和AA对肝癌细胞的作用有协同性，他们通过caaspase-3而不是ERKs的激活进一步抑制了肝癌的生长，诱导其凋亡。

RESULTS:(1) ET-1 could increase total protein production, surface area, ERKs activity and ［Ca2+］i in cultured cardiomyocyte in dose-dependent manner at concentrations ranging from 10-9 to 10-7 mol/L. And this effect could be abolished by BQ123, an antagonist of ETA receptor, partly inhibited by PTX, but not by BQ788, an antagonist of ETB receptor.（2）The activation of ERKs and the increase of ［Ca2+］i induced by ET-1 were obviously inhibited by PD98059, a selective ERKs kinase inhibitor, and nifedipine, a calcium channel blocker, respectively. Both antagonists partially inhibited ET-1-stimulated cardiomyocyte hypertrophic response.(3) Staurosporine, a selective PKC inhibitor, could inhibit ET-1-stimulated cardiomyocyte hypertrophic response and increase of ［Ca2+］i, but not affect the activation of ERKs.

结果： ①ET-1浓度依赖性增加新生大鼠心肌细胞蛋白质含量和心肌细胞表面积、ERKs活性及［Ca2+］i浓度，以上作用可被ETA受体拮抗剂BQ123所完全抑制，被百日咳毒素部分抑制，而ETB受体拮抗剂BQ788则无效；②ERKs激酶特异性抑制剂PD98059可完全抑制ET-1激活ERKs的作用，钙通道拮抗剂硝苯地平可明显抑制ET-1介导的［Ca2+］i浓度增加，但二者皆仅部分抑制ET-1介导的心肌细胞肥大反应；③蛋白激酶C选择性抑制剂staurosporine并不能明显抑制ET-1介导的ERKs激活，但可抑制ET-1介导的的［Ca2+］i浓度增加及心肌细胞肥大反应。

METHODS: Under the same conditions, the samples of titanium alloy and CFR/PEEK were placed in the fresh human blood so as to determine blood compatibility via haemolysis rate, platelet activation level, and leukocyte activation level.

在相同条件下，将钛合金和碳纤维增强聚醚醚酮2种材质的试件分别放置于新鲜人血中，通过观察溶血率，血小板激活程度以及白细胞激活程度判断材料的血液相容性。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷