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The result of frozen MⅡoocytes using three methods indcated that the self-restraintspear method was better than OPS, and the effection of frozen altogether with self-restraintspear method and centrifugal was the best; the frozen effection was not ideal using fluidspear for 8- cell ovocyte.

用三种冷冻方法(OPS法;自制移液枪头法;移液枪头法+离心法)对MⅡ卵母细胞冷冻的结果表明,自制移液枪头法优于OPS法,其中以移液枪头法+离心组的冷冻效果最好;用移液枪头法对体外发育到8-细胞期卵母细胞的冷冻效果不理想。MⅡ期卵母细胞用OPS法冷冻后卵母细胞IVF和电激活的卵裂率都显著高于移液枪头法(P<0.01),电激活后2-细胞卵裂率比IVF高。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性,最适作用温度为42℃;最适pH值为9.0;Mg2+、Ca2+对此酶有明显的激活作用,而Cu2+能完全抑制酶的活性;174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性,初步说明此酶属于丝氨酸蛋白酶类;体外溶纤作用表明,该酶溶解纤维蛋白的方式是直接溶解,而不是通过激活纤溶酶原。

When the activity of MAPK was sustained after electronic parthenogenetic activation, the activity of 〓 was also maintained.

放线菌酮不抑制GVBD的发生,但抑制MAPK的激活,此时〓也不被激活

Inactivation of 〓 began to take place at the time correlated with the completion of MAP kinase inactivation after parthenogenetic activation.

在IBMX的作用下MAPK和〓都不能被激活,但在IBMX+OA作用时MAPK和〓都可被激活

A23187 and OA could parthenogenetically activate Urechis unicinctus oocytes and activate MAPK, indicating that the effect of fertilization on GVBD and activation of MAPK was mediated by rising in intracellular free calcium, and phosphorylation of some proteins was enough to activate the oocytes.

钙离子载体A23187和OA可以孤雌活化单环刺螠卵母细胞并激活MAPK。说明:①受精是通过钙离子的内流来激活卵母细胞并使MAPK活化的。②某些蛋白质的磷酸化状态对减数分裂是必要而且在一定程度上是充分的。

Urokinase-type plasminogen activator is a very important serine protease, which is best known for its role in the initiation of the pericellular proteolystic cascade.

尿激酶型纤溶酶原激活剂(Urokinase-Type Plasminogen Activator,uPA)是—种非常重要的丝氨酸蛋白酶。uPA不仅本身是一种蛋白酶,更重要的是,uPA能够激活一系列蛋白酶,降解细胞外基质。

Results: It indicated that compared with the one character naming task, the brain activation regions were smaller and more concentrated in three character naming task, and more deactivation was appeared in the left pericentral gyrus, right precentral and postcentral gyrus, left superior temporal gyrus and right putamen.

结果:与单字命名条件相比,三字命名条件下的脑激活范围更小、更集中。而且,三字命名条件在左侧中央旁回、右侧中央前回和中央后回、左侧颞上回以及右侧壳核出现了更强的负激活

Increased phosphorylated ERK1/2 in retinal Müller cells by using an intravitreal glutamate insult suggests that phospho ERK1/2 plays an important neuroprotective role when retinal ganglion cells are injured by glutamate cytotoxity.

玻璃体内注射谷氨酸导致大鼠视网膜Müller细胞激活,ERK1/2磷酸化水平增高,ERK1/2通路的激活可能在视网膜节细胞受到兴奋性毒性损伤时起到重要的保护作用。

Intracytoplasmic sperm injection, which is a new technique of embryo engineering of domestic animals, can solve the problem of porcine polyspermy.

用GFP基因转染的精子注射后化学激活的ICSI卵母细胞基因表达率显著高于电激活处理的ICSI卵母细胞的基因表达率。

Results:In comparison with fixation cross, recognition of neutral faces activated the left middle frontal gyrus, the bilateral precentral gyrus, the right amygdala, the left parahippocampal gyrus, the right postcentral gyrus and the left thalamus.

图像数据经SPM2软件处理和统计分析,获得脑区激活图。结果:与识别十字架相比,识别中性表情激活左额中回、双侧中央前回、右侧杏仁核、左顶下小叶、右中央后回以及丘脑等。

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I urge any American living outside the country to get an early start checking his/her registration and receiving an absentee ballot, because it can take a while.

我奉劝身在国外的所有美国人早点着手核对登记表,并接收缺席选举人票,因为这得花点时间。

He is too young to join the army.

他太年轻了,还不能参军。

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