激光诱导荧光

First the traditional non-optical detection technology for gas concentrations is briefly introduced, such as ultra-sound technology, gas sensing, catalytic thermal, gas chromatography, interferometry applications, and then the spectral analysis based on the concentration of gas detection is discussed. The latest technological developments on the differential absorption spectroscopy, Fourier transform infrared spectroscopy, tunable laser diode laser absorption spectroscopy, laser radar and differential absorption Raman scattering laser radar, laser-induced fluorescence spectroscopy, laser Photoacoustic Spectroscopy are reviewed in detail.

首先对传统的非光学气体浓度检测技术作出了简单的介绍，包括超声波技术、气敏法、热催化法、气相色谱法、干涉法应用技术，被动检气管法，然后重点阐述了基于光谱学分析气体浓度检测技术的最新发展动态，其中分别对差分吸收光谱技术、傅里叶变换红外光谱技术、可调谐激光二极管激光吸收光谱技术、差分吸收激光雷达和拉曼散射激光雷达、激光诱导荧光光谱技术、激光光声光谱技术进行了详细介绍，最后提出了现代气体浓度检测技术的发展方向。

The laser induced fluorescence and its derivative spectra of dysprosium europium, samarium, and terbium in the system of TFATOPO-Nongru-100 was studied and the method for the simultaneous determination of the four rare earths was developed.

我们利用自己编写的数字微分软件，研究了TFA-TOPO-农乳-100体系中Dt、Eu、Sm和Tb的激光诱导荧光及其导数荧光光谱，拟定了同时测定四个稀土元素的分析方法。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

We found that luminescent CdTe/CdS QDs prepared in aqueous phase very facilely conjugated to antibodies based on their strong adsorption.

运用毛细管电泳-激光诱导荧光检测、荧光光谱法和荧光相关光谱法对量子点-抗体标记产物进行了分析。

Based on studies about current development of Methods of separating meiofauna, a new method is advanced in this paper. A suit of device was designed to Separate meiofauna from benthic sediments sample automatically, accurately and efficiently, which based on the technology of Laser-induced Fluorescence Detection, and used other technologies, such as noctilucence, electronics, biophysics, computer, mechanism, and so on. The scheme of macrocosm, the design of light route, the hardware and software design of the photoelectricity detection system and machine control system, the method of signal processing are introduced, especially the hardware and software design and the method of signal processing.

论文分析了目前国内外所使用的小型底栖生物分离方法现状，并以此为基础，自主开发了一套基于激光诱导荧光技术，并且集荧光化学技术、电子物理技术、光电测量技术、计算机技术及机械技术为一体的小型底栖生物标本自动分离系统，实现了从沉积物样品中自动分离小型底栖生物标本，从而大幅度提高室内小型底栖生物分样的工作效率和精度的目的，并以此完成了大洋协会"十五"攻关项目："小型底栖生物标本自动分离系统研制"。

In the work, the laser-behavior under exposure of Ar〓 laser was discussed. The kinetic process of photochemical reaction, intermediate, sensitive process of diazophthoquinon system were studied by laser fluorescence spectrum and laser induced fluorescence attenuation. The nonlinear relation between photodecomposition rate of the system and laser power and concentration of solution was investigated in detail.

论文也进行了感光组成物感激光性能的研究：利用激光荧光光谱及激光诱导荧光衰减法实现了实时探测邻叠氮萘醌型光敏剂感激光光化学过程、中间体的产生与结构以及增感作用的研究，该方法在国内外文献中未见有报道。

By means of time-resolved laser-induced fluorescence spectroscopic technique, two samples of Er3+∶YAG with 10.5% and 8.4% concentrations were resonantly excited with ultraviolet lasers at 262 nm and 276 nm, and the fluorescence spectra were recorded and the peak transitions were assigned in detail.

利用时间分辨激光诱导荧光光谱技术，用262 nm和276 nm紫外激光对原子数分数为x(Er3+)=10.5%和x(Er3+)=8.4%两种情况下Er3+∶YAG晶体的4D5/2和2H9/2能级分别共振激发，记录了每种情况下的荧光发射谱，对其中所有荧光谱峰对应的能级跃迁做了详细标定。

On the Mechanism of Populating d~3∏ State of NaK by LIF Method;2. We apply the technology of LIFto diagnose hepatic fibrosis which is induced by different matter.

本文应用激光诱导荧光的方法，对不同物质诱发白鼠的肝纤维化的肝组织进行了荧光光谱分析，发现经过注射不同物质的白鼠的肝组织与正常白鼠的肝组织的荧光光谱有明显不同。

Based on this,first part of this work has established spectrophotometer and laser induced fluorescence spectrographic methods based on the liquid-core waveguide,and made it successfully apply to detecting iron and the cupric ion concentration.

基于此，本工作第一部分建立了基于液芯光纤的分光光度法和激光诱导荧光光谱法，并成功地应用于1，10邻二氮菲对铁的检测和催化荧光动力学方法对铜离子浓度的测定。

Using Rose Bengal as protein dye, based on the fluorescent dye spectrum experiment and the modeling of system signals, A laser-induced fluorescence detecting system for marine meiobenthos in the sediments is set up.

设计小型底栖生物标本荧光检测方案，通过荧光染料虎红光谱实验和激光诱导荧光检测系统光电信号模型分析，建立实验系统。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。