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In the titration experiment of NMR and IR spectroscopy, we can understand how sensors binding with metal ions: the two carbonyl group and crown ether group cooperated in binding with metal ions. Furthermore, by altering the "oxygen" atoms of crown ether to "sulfur", we can change the binding affinity to the soft metal: cupper. It shows 60-fold enhancement of fluorescence.

在化合物2中,我们引进了硫原子在冠状醚中,则应对电子密度较低之金属有较好的结合能力,实验结果显示了此分子对铜离子有相当好的选择性和结合能力,萤光增加可达60倍;同时由红外光吸收光谱和核磁共振光谱滴定实验可确知,香豆素衍生物上的两个羰基对金属离子的选择性及结合上扮演相当重要的角色。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果:1、通过不同感染途径的实验证实,滴鼻感染的小鼠主要表现为间质性肺炎,而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现,随着感染Q热立克次体剂量的加大,动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现,在腹腔注射后第2d的脾和肝脏即可发现病原体,主要脏器的病理变化从第2d到第12d逐渐加重,第7d动物的病变最典型,至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统,在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内,呈多形性,多见圆形和杆状,大小不一。

Two alloying platings composed of same basic component for both deposited layer and the substrate , prepared by non-magnetron ion plating with arc source process, are analyzed with scanning electron micro.

对QA19-4/Cu及1Cr18Ni9Ti/45钢等两类同基合金的非磁控电弧离子镀镀层进行的扫描电镜分析结果表明,由于弧流携带的液滴较多,沉积层组织呈细密的片层状,层厚2-3μm。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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