滴流

To develop a rapid and accurate method for baculovirus titration, flow cytometry was adopted to quantitate baculovirus directly after staining with SYBR Green I.

为了快速而准确的测定杆状病毒的滴度，采用流式细胞术对经SYBR Green I染色后的杆状病毒直接计数。

To study the surface hydrophobicity of solid materials with the lattice Boltzmann method , the rough surface is approximately treated with simple periodic rectangular, saw-toothed and semicircular protrusions structure respectively. A two-phase lattice Boltzmann model is used to simulate the changes of surface hydrophobicity by calculating the contact angles of a droplet on rough surfaces.

将固体表面分别近似为具有简单的周期性矩形、三角形和半圆形微粗糙结构表面，建立两相流的晶格玻尔兹曼模型，通过测量不同微粗糙结构表面上液滴的接触角，探讨微结构形状和尺寸的改变对固体材料表面疏水性能的影响。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

My days are dripping into the stream of time, soundless, and traceless.

我的日子滴在时间的流里，无声无息，不见踪迹。

Disappearing into the ocean, my days are dripping into the stream of time, soundless, traceless.

我的日子滴在时间的流里，没有声音也没有影子。

Stream of time, soundless, traceless.

子滴在时间的流里，没有声音也没有影子。

I don't regret spilling a single drop of it.

我不在乎他流的每一滴血。

Behold, the days come, saith the LORD, that the plowman shall overtake the reaper, and the treader of grapes him that soweth seed; and the mountains shall drop sweet wine, and all the hills shall melt.

9：13 耶和华说，日子将到，耕种的必接续收割的，踹葡萄的必接续撒种的。大山要滴下甜酒，小山都必流奶（原文作消化，见珥03：18）。

Behold, the days come, says the LORD, that the plowman shall overtake the reaper, and the treader of grapes him that sows seed; and the mountains shall drop sweet wine, and all the hills shall melt.

耶和华说：日子将到，耕种的必接续收割的；踹葡萄的必接续撒种的；大山要滴下甜酒；小山都必流奶（原文是消化，见约珥三章十八节）。

Behold, the days come, saith the LORD, that the plowman shall overtake the reaper, and the treader of grapes him that soweth seed; and the mountai shall drop sweet wine, and all the hills shall melt.

9：13 耶和华说，日子将到，耕种的必接续收割的，踹葡萄的必接续撒种的。大山要滴下甜酒，小山都必流奶（原文作消化，见珥03：18）。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。