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Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上,利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中;对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

7TM phage display peptide l ibrary was biopanned for 4 rounds to harvest monoclonal anti-body human KGF and then phage tilter was detected.

以人KGF 单克隆抗体为靶,对噬菌体随机七肽库进行4 轮生物淘选,测定噬菌体滴度

This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.

构建成功的新型慢病毒载体为今后的基因治疗奠定了基础,也为慢病毒滴度的确定提供了一种新的可能。

Objective To investigate the relationship of human immunoglobulin and complement with both the type and titer of antinuclear antibody.

目的探讨抗核抗体核型及滴度与免疫球蛋白和补体的关系。

Lymph nodes of the extent and level of serum antibody titer of AIDS-related.

淋巴结肿大的程度与血清内艾滋病抗体滴度高低相关。

Several methods are available for measuring the titer or level of virus in serum, which is an indirect assessment of viral load.

几种方法可用於测量水平的滴度或血清中的病毒,这是一个间接的评估病毒载量。

Results HA titer of two Lanzhou strains cultured in chicken embryo was high up to 1∶512.

结果 2株兰州株在鸡胚中培养血凝效价滴度高达1∶512。

The titer of antibody in 15 sera samples of them were reaching 1:1280 or more.

值得注意的是,在65例以前患过EHF的病人中,有35例发现特异荧光,其中有两人是196O年的患者,但其抗体滴度低于1:320。

Objective To find factors affecting HBeAg titer and serological status.

目的:分析病毒和临床因素对HBeAg血清学状态和滴度的影响。

Results The titer of lentivirus was 6×10^5IU/mL, transduction efficiency ranged from 6~11%.

结果 包装的慢病毒滴度达6×10^5IU/mL,对J558L细胞的感染效率为5%~10%。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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