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Methods Sindbis virus, Pseudorabies virus and poliovirusl (PV1) were used as indicated viruses in this test.

将Sindbis病毒、伪狂犬病毒和脊髓灰质炎病毒(PV1)3种指示病毒分别加入不同的UTI原料样品中,进行60℃水浴10h和乙醇处理3h,处理不同时间后分别取样,用微量细胞病变法检测不同时间段样品中的病毒残留滴度

To develop a rapid and accurate method for baculovirus titration, flow cytometry was adopted to quantitate baculovirus directly after staining with SYBR Green I.

为了快速而准确的测定杆状病毒的滴度,采用流式细胞术对经SYBR Green I染色后的杆状病毒直接计数。

Methods Culture poliomyelitis virus types Sabin Ⅰ and Ⅱand Zhong Ⅲ2 in KMB17 cells by suspension adsorption and adding serum.

应用悬浮吸附和添加血清的方法,在KMB17细胞上培养SabinⅠ、Ⅱ、中Ⅲ2型病毒,用微量法检测病毒滴度

As a result,the time course of the antibody titers to both I and II were parallel to that of macroscoopic and microscopic observations in an experimental mouse sporotrichosis model.

结果显示抗体滴度与实验小鼠孢子丝菌病肉眼和镜下观察的变化趋势相一致。

BMSCs expressing Ad-GFP had three types of subpopulation structures under a fluorescence microscope; when the virus titer was ≥ 1×10^6 PFU/mL, BMSCs proliferation was suppressed at the early stage, in a dose-dependent manner.

荧光显微镜下可见表达Ad—GFP的BMSCs有三种亚群结构:滴度≥1 10^6PFU/mL时,BMSCs增殖在早期受到抑制,且呈剂量依赖;转染Ad—GFP的BMSCs经β一巯基乙醇诱导可分化为神经元样细胞,神经元特异性烯醇化酶阳性。

Patients typically have an elevated anti- streptolysin O titer.

患者抗链球菌溶血素 O 滴度显著增高。

The model of HCC was established by injecting subcutaneously the cells in different groups in nude mice.

结果 重组腺病毒AdEasy-GFP-ASmrp滴度可达2.5×10〓efu/ml,MOI为100时,即可致90%以上的肝癌细胞SMMC-7721/ADM被感染。

In the present paper, the induction characteristic of lymphoblastoid, in-terferon in vitro is described. Results indicate that Namalwa cell line possesses a higher interferon-inducing ability than Raji and Yu-Nu cell lines. NDV B1 strain, F strain and Sendai virus can induce more interferon than BB1 virus and poly 1 : C. Priming of pretreating cells with homologous interferon or superinduction did not effect interferon production. The optimal cell concentration for interferon induction is about 106 cells/ml...

本文研究了类淋巴母细胞干扰素的诱导特性:Namalwa细胞系的干扰素诱生能力大于Raji和余奴系,而诱生剂中以NDV和仙台病毒诱生干扰素的能力较大,但"起动"和超诱导均无作用;诱生干扰素适宜的细胞浓度为10~6/毫升;增殖期的Namalwa细胞诱生能力较大;黄芪和5'-溴脱氧尿嘧啶核苷对Namalwa细胞干扰素的诱生系统有明显刺激作用,在最适条件下粗制干扰素平均滴度可达40,000单位/毫升。

In the present paper, the induction characteristic of lymphoblastoid, in-terferon in vitro is described. Results indicate that Namalwa cell line possesses a higher interferon-inducing ability than Raji and Yu-Nu cell lines. NDV B1 strain, F strain and Sendai virus can induce more interferon than BB1 virus and poly 1 : C. Priming of pretreating cells with homologous interferon or superinduction did not effect interferon production. The optimal cell concentration for interferon induction is about 106 ce...

本文研究了类淋巴母细胞干扰素的诱导特性:Namalwa细胞系的干扰素诱生能力大于Raji和余奴系,而诱生剂中以NDV和仙台病毒诱生干扰素的能力较大,但"起动"和超诱导均无作用;诱生干扰素适宜的细胞浓度为10~6/毫升;增殖期的Namalwa细胞诱生能力较大;黄芪和5'-溴脱氧尿嘧啶核苷对Namalwa细胞干扰素的诱生系统有明显刺激作用,在最适条件下粗制干扰素平均滴度可达40,000单位/毫升。

The effect of AAV vector on IPE cells'growth was determined by methyl thiazoly tetrazolium assay.

MTT法显示:在不同病毒滴度的转染组,IPE细胞生长的OD值与未转染组比较无统计学差异。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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