滴度

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Determine the virus titer by microtitration and plaque formation test and the virus proliferation by IFA, and perform genomic stability test.

用微量滴定法与蚀斑法平行检测病毒滴度，用免疫荧光法检测病毒的增殖情况，最后进行病毒基因组稳定性检测。

These Cox.B prototype viruses were titrated on both Hep-2 and L-hCAR cells and their titers were similar.

B 1～6型标准株进行滴定，两种细胞上的病毒滴度无显著性差异。

Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

CF titres are expressed as the reciprocal of the serum dilution producing 50‰ haemolysis.

以产生50%溶血的最终血清稀释度的倒数表示补体试验的滴度。

It took about 5 days for all cells of primary bovine testis to be infected by CSFV and the titre of progeny virions in medium was 10 5 F PFU/ml from then on.

使用改进的病毒滴度测定方法，对接毒后培养上清中病毒滴度的变化趋势进行检测。

Poor responders with titres of 10 to 100 miu/ml should have a booster and those with a titre below 10 miu/ml should repeat the course.

对10 to 100 miu/ml滴度反应不明显的人应该打加强针，对小于小于10 miu/ml滴度的人应该重复注射

After three biopanning procedures , there was remarkable enrichment for the titer of bound phages in each biopanning procedure.

本研究共进行三轮生物淘洗，滴定每轮产出噬菌体的滴度，计算噬菌体的产率。

Results In each stage of follow-up,the RPR test titer descended more than 2 titers was 48.6% for 3 months of treatment,78.4% for 6 months and 94.6% for 9 mon...

结果治疗后3个月血清RPR试验滴度下降2个以上稀释度的比例为48.6%，6个月时为78.4%，9个月时为94.6%，12个月时RPR试验转阴率达83.8%。

Methods: Nine patients with severe systemic lupus erythematosus were treated with DNA immunosorbent column 3 times respectively, and parameters such as the titer of anti-nuclear antibody, anti- double-stranded DNA antibody titer, Complement C3, erythrocvte sedimentation rate, routine test of blood, kidney function and liver function were observed before and after the treatment, the results were analyzed statistically.

应用DNA免疫吸附柱对9例重型系统性红斑狠疮患者分别治疗3次，观察吸附治疗前、后抗核抗体滴度抗双链DNA抗体滴度、补体C、血沉、血常规、肾功能、肝功能等指标并行统计学分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。