滴度

Abstract］ Objective For the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

Abstract］ objective for the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.methods aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with ph 4, human immunoglobulin and human hepatitis b immunoglobulin for intravenous injection with ph 4/pasteurization,treatment on human fibrinogen and human coagulation factor ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.the mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.results human albumin pasteurization inactivated vsv and sindbis virus,hrig low ph inactivated vsv and sindbis virus,hrig low ph inactivated hiv virus,ivig low ph/pasteurization inactivated hiv virus,ivig low ph inactivated hiv virus,hbig low ph/pasteurization inactivated vsv,sindbis and polio virus.conclusion suggestion about improvement on unreasonable parameter and standard of virus inactivation is made,cultivate target virus directly from final product by appropriate cell or method to monitoring viral safety of blood plasma product is advanced.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低ph常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低ph/巴氏消毒法；人纤维蛋白原、人凝血因子ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。结果人血白蛋白巴氏灭活vsv与sindbis病毒，hrig低ph灭活vsv和sindbis病毒，hrig低ph灭活hiv病毒，ivig低ph/巴氏灭活hiv病毒，ivig低ph灭活hiv病毒，hbig低ph/巴氏灭活vsv、sindbis与polio病毒。结论建议改进病毒灭活验证标准与不合理的灭活参数；采用终产品样品通过合适细胞或方法直接培养目标病毒来有效监测血液制品的病毒安全性。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

The accordance rate of the two methods was 98.8%. Conclusion: To reduce the harm of syphilis to mothers and infant, we should generalize the combined method of the specific and nonspecific serology tests, and do TRUST titer test when positive.

为降低梅毒对妇婴的危害，应推广特异性血清学试验与非特异性血清学试验联合做为筛查，阳性的加做TRUST滴度。

SiRNAs were tested for silencing expression of the major capsid protein encoded by tiger frog irus, an iridoirus causing seere disease in fish. siRNAs targeting mcp gene effectiely inhibited TF replication in fish cells as demonstrated by reduced mcp RNA leel, postponed emergence of cytopathogenic effect, as well as reduced TF titer and particles in cells.

TF的MCP基因编码一个主要衣壳蛋白，通过转染针对MCP基因的siRNAs能减少了MCP基因RNA水平，从而沉默MCP基因表达；在FHM细胞上延迟了CPE的出现，降低了TF的滴度和病毒粒子的数目。

In this study,the strain CH-la isol ated from china was used as vaccine virus,the rolling bottle technique was adopted to propagate higher titer PRRS virus on Mare-145 cell lines.

本研究选用国内分离的猪繁殖与呼吸综合征病毒CH-1a株作为疫苗用毒株。利用转瓶细胞培养技术，在Marc-145细胞上大量增殖了较高滴度的猪繁殖与呼吸综合征病毒。

Results The recombinant plasmid pSNAV2.0-TK was verified byPCR and restricton endonuclease. The coat protein of AAV was detectedby SDS-PAGE method, the purity of rAAV2/HSV-TK was＞98% byHPLC method and the titre of rAAV2/HSV-TK was 1×10~(12)v.g./ml byDNA dot blot hybridization.

结果：重组质粒pSNAV2.0-TK PCR扩增的片段大小和酶切鉴定所切下的片段与预计结果一致；SDS-PAGE法检测到三条特征性的AAV外壳蛋白条带，HPLC法检测rAAV2／HSV-TK的纯度＞98％；用DNA斑点杂交方法检测rAAV2／HSV-TK的滴度为1×10~(12)v.g。

Results: The titer of Ad-PKG Ⅱ was 5×l0^9 pfu/ml. The PKG Ⅱ protein expression in Ad-PKG Ⅱ infected CS-54 cells was significantly increased in comparison with that in the control cells; in gastric cancer line BGC-823, PKG Ⅱ significantly inhibited the EGF induced activation of ERK.

结果：重组腺病毒滴度达5×10^9pfu/ml，蛋白质印迹法检测显示重组腺病毒感染CS54细胞后使其PKG Ⅱ表达明显增高；在BGC-823胃癌细胞株，PKG Ⅱ对EGF导致的ERK的激活有明显抑制作用。

The results showed that there were two positive-antibody parachutists with the titer 1∶10(positive rate was 5%)in control and there were four positive ones with 1∶10(positive rate was 9.6%)including two with 1∶40positive rate w...

结果：对照组40人中有2人出现1∶10阳性，占5％，而跳伞组42人中4人出现1∶10阳性，占9.6％，其中2人1∶40阳性，占4.8％。结论：高滴度的HSP70抗体出现可能是空降兵热敏感和免疫功能混乱的综合反映

Parachutists Parachuting Heat stress protein Antibody titer Enzyme linked immunosorbent assay

空降兵；跳伞；热应激蛋白；抗体滴度；酶联免疫吸附试验

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。