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To induce high levels of neutralizing antibodies is one of the obstacles in HIV-1 vaccine development.

此外,在体内诱导出高滴度的中和抗体是HIV疫苗研究的一个难题。

After 1 week's treatment, the patients received individually titrated nasal allergen challenges once every morning for 8 days while treatment continued.

治疗1周后,在治疗同时每天早晨对患者给予个人滴度的鼻变应原进行激发,持续8天。

Methods Model of Guinea pig genital herpes was made and treated,with the anti-virus capsule.The treatment effect and mechanism were estimated with vulvae symptom grade and vaginal secretion virulence.

方法先进行豚鼠生殖器疱疹造模,然后用中药抗病毒胶囊对生殖器疱疹豚鼠进行治疗,用外阴症状评分和阴道拭子病毒滴度进行疗效的评价和机理的探讨。

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种,接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况;并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

Induction of hepatoma cells migration by phosphoglucose isomeerase/autocrime motility factor through the upregulation of matrix metalloproteinase-3.

本研究中,我们在RA患者血清和关节液中发现了高滴度的G6PI抗原,明显高于正常对照组和其它结缔组织病组。

The livers of sd bandicoots were infected by raav. at the 2nd month and 9th month, southern blot assay and dot blot assay were used to detect the integrated gene and mrna of hcv core protein and the titer of the raav respectively.

重组腺伴随病毒感染大鼠肝脏,感染2, 9 mo后分别以southern blot印迹杂交法和dot blot杂交法检测鼠肝hcv core区基因的整合、mrna形成情况及病毒滴度

Results We obtained a cDNA library of ciliates Euplotes, the titer of which was -2.437×107cfu/mL, which could suffice for functional gene screen.

首次得到了可用于筛选功能基因的原生动物纤毛虫的cDNA文库,文库滴度为2.437×107 cfu/mL。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein, Ad-Math1-EGFP)前庭阶导入组,每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater, CDM-CEP)、听性脑干反应阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。

By means of co-precipitation with calcium phosphate,the recombinant pLHC2547 was transfected into PA317 cells.The lines were screened with G418 and the virus titer in supernatant of the colones was determined by NIH3T3 cells.

通过磷酸钙-DNA共沉淀法把经鉴定的重组体转染入PA317细胞,用G418进行克隆筛选,以NIH3T3细胞测其病毒滴度

By means of co-precipitation with calcium phosphate,the recombinant pLHC2547 was transfected into PA317 cells.The lines were screened with G418 and the virus titer in supernatant of the colones was determined by NIH3T3 cells.

通过磷酸钙-DNA共沉淀法把经鉴定的重组体转染入PA317细胞疗效,用G418进行克隆筛选,以NIH3T3细胞疗效测其病毒什么滴度

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