滴度

To clarify the acetylcholinesterase changes among different developmental stages of Tribolium castaneum, the toxicological and biochemical characterizations of AChE in 10-d larvae, 20-d larvae, pupae and adults were investigated, respectively with the microplate reader method.

为了阐明赤拟谷盗体内乙酰胆碱酯酶在其各虫态间的变化关系，以期为该虫的综合治理提供一定的理论依据，本试验采用微量滴度酶标板法，分别对其10d龄和20d龄幼虫、蛹及成虫体内AChE的生化及毒理学特性进行了较为系统的研究。

The titer of cDNA library unamplified was 1.02×10^6 pfu/mL, and the recombinant rate was 95.3%.

未扩增文库的滴度是1.02×10^6 pfu/mL，重组率达95.3%。

Analysis of the cDNA library suggested that the titer of unamplified library was 1.1×106 pfu/mL, and the average size of inserts was about 750 bp.

原始文库的滴度为1.1 ×106 pfu/mL，平均插入片段长度为750 bp。

Results showed that the transgenic plants displayed slight or no symptom. The average PLRV titer in the transgenic lines was lower than the untransformed plants.

结果表明，表达PLRV IS正意和反意RNA的转基因植株，接种病毒后表现无症状或症状轻微，PLRV平均滴度均较未转基因对照植株低。

Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of culture, cell density can reach 6~7×106/mL, then rabies virus VaG strain was inoculated with a MOI of 0.01. After 24h inoculation, the cultured viruses were harvested continuously for 12 days. The titer of collected virus in the culture medium was about 6.0 to 8.5 logLD50/mL. From these collected viruses, vaccines were prepared through the steps of concentration, inactivation and purification, The quality of these vaccines meets "The Chinese Requirements for Biologics Year 2000".

在5L体积的生物反应器中，加入含10g/L微载体的199培养基，接种Vero细胞至细胞浓度达到1×105/mL，培养7d后细胞可生长至6~7×106/mL，然后以感染复数为0.01接种狂犬病毒VaG株，接毒后24h开始收获，连续收获12d左右，收获的病毒滴度范围在6.0~8.5logLD50/mL，收获的病毒原液经浓缩、灭活和纯化等步骤制备成疫苗，各项质量指标均达到《中国生物制品规程》2000年版要求。

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化，病毒最终滴度达到109 PFU/ml；3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长，这与本课题组在在体动物实验中发现的现象一致。

Using indirect ELISA, the contents of vitellin in the fat body, haemolymph and ovary of P. japonica were detected. The results suggested that the vitellogenin was synthesized in fat body at the 2nd day after eclosion. The contents of vitellogenin in fat body, haemolymph and ovary increased quickly on the 4th day after eclosion and reached the peak stage approximately on the 8th day in adult stage.

采用间接竞争ELISA法，系统测定了龟纹瓢虫成虫期脂肪体、血淋巴和卵巢中卵黄蛋白的动态变化，结果表明：脂肪体是卵黄原蛋白合成的场所，卵黄原蛋白的合成始于羽化后第2天；脂肪体、血淋巴和卵巢中卵黄原蛋白的滴度在羽化后第4天开始迅速上升，至成虫期的第8天左右达到高峰期。

To inestigate sensitisation against food antigen, mice were intragastrically administered with oalbumin eery other day for nine weeks, and antioalbumin antibody titres were measured weekly.

为了研究对食物抗原的致敏反应，小鼠予胃内灌注卵白蛋白，隔天1次，共9周，每周测试卵白蛋白抗抗体滴度。

The two reconstructed plasmids were packed to infective virus particles in incasing cells PT67 with a tite of about 5. 8×10〓-3. 4×10〓cfu/ml.

2两种重组表达质粒在PT67细胞中包装成具有感染能力的病毒颗粒，产生病毒滴度在5.8×10〓-3.4×10〓cfu／ml之间。

The results showed that the first sixth risk factors were the rate of carrying meningococci A and B in the population,relative air humidity in January,morbidity rate in January,vaccination rate before epidemic,and the titre of antibody.

结果表明，影响流脑流行的前六位因素是：人群流脑菌B群、A群带菌率、一月份相对湿度、一月份发病率、流行前期流脑接种率和人群抗体平均滴度。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。