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It was confirmed through passive immunization of IgY that healthy nonimmunized rabbits whose weight varied during 2Kg and 2.5Kg were intramuscularinjected with IgY 5.0ml (HI=13log2),they could resist the attact of 1.0ml(HA=9log2) RHDV-RC.

通过IgY的被动免疫保护试验证实,IgY滴度稀释至13log2时肌肉注射体重2Kg-2.5Kg的健康非免家兔5.0ml/只,可抵抗兔病毒性出血症强毒RC株1.0ml(血凝价HA为9log2)的攻击。

The maximum titer of this preparation reached 15log2 by hemagglutination inhibition assay.It was confirmed through passive immunization of IgY that healthy nonimmunized rabbits whose weight varied during 2Kg and 2.5Kg were intramuscularinjected with IgY 5.0ml (HI=13log2),they could resist the attact of 1.0ml(HA=9log2) RHDV-RC.

通过IgY的被动免疫保护试验证实,IgY滴度稀释至13log2时肌肉注射体重2Kg-2.5Kg的健康非免家兔5.0ml/只,可抵抗兔病毒性出血症强毒RC株1.0ml(血凝价HA为9log2)的攻击。

Results No post-inoculation pyrogenetic reaction were observed in both trial group and control group, the local reaction rates being 1.8% and 2.1%(P〉0.05), respectively, indicating slight reactions.

方法按整群随机抽样原则,以进口同类疫苗作为对照开展现场临床试验;比较两种疫苗免疫后临床反应率、抗体阳转率、保护率及几何平均滴度

objective to investigate the infection of mycoplasma pneumoniae in children.methods the glutin agglutination rection was used, the diluting power of antibody of mycoplasma pneumoniae was more then 1:80, it was a positive sample.results 1 817 patients were detected, the positive rate was 30.7%,the female rate was higher than male rate, the highest rate were between three to six years old.conclusion mycoplasma pneumoniae is the main pathogen in children with respiratory tract infection, the positive rate was connected with age and nearly contact each other.

目的 了解肺炎支原体在青少年中的感染情况。方法采用明胶凝集的方法,若抗mp-igm滴度≥1:80,为阳性。结果共检测1817例,阳性率为30.7%,女性阳性高于男性,3~6岁的儿童阳性率最高。结论肺炎支原体的感染是青少年呼吸道感染的重要病原菌,阳性率与年龄有关,密切接触可引起局部流行。

After 3 inoculations,the mice were sacrificed and their splenocytes and sera were collected.T cell subsets were analyzed by FCM.IFNγ in their cultural supernatants and seral IgG antibody against CEA were detected with ELISA.

FCM分析脾脏T细胞亚群;体外培养脾细胞,ELISA检测培养上清中干扰素γ的相对含量;同时测定小鼠血清CEA特异性抗体的滴度

Meanwhile many cases were diagnosed to be sero positive of anti dengue virus. Dengue I virus were also isolated from five cases. RT PCR test also indicated 4 out of 8 samples contained dengue I virus, which was the same type of dengue virus found in the epidemic areas in Guangzhou at the same time of outbreak.

进一步的调查在病例的血标本中分离出5株登革I型病毒,同时用RT—PCR的方法从8份标本中检出4份登革I型病毒阳性,与同期广州市登革热流行的病毒型别一致;取患者的恢复期血清作肥达氏反应,结果抗&O&、&H&的滴度与第一份血相比,均显著下降。

These results denoted that binding to sialic acid may be useful for IBV toinfect cells.

以前的研究证实胰酶可以提高某些病毒的滴度

Methods Mix appropriate of the negative and positive in the box by looking at serum, compare the differences between different reagents and CO values of differents titers, screen out the suitable blending ratios, then subpackage、refrigerate、preserve to use.

利用试剂盒内的阴、阳性对照血清按一定量混合,通过比较不同试剂、不同滴度的CO值,筛选出合适混合比例,分装冷冻保存使用。

Key words] Thyroid peroxidase antibody;Autoimmune thyroidism disease;Factors inducting into disease

性别、年龄、血清TPOAb滴度均与血清甲状腺过氧化物酶抗体阳性患者甲状腺疾病产生有一定的相关性。

A cDNA library with a tittering of 7+106 pfu/mL was constructed basing on the cotton boll shell (10 days post anthesis, dpa) RNA obtained above. The recombinant ratio was found to be ca. 90% by blue-white counting.

首次构建了构建了花后10天棉铃壳cDNA文库,未稀释文库滴度达到7×10~6,蓝白斑计数计算重组率为90%,为棉铃发育基因及其启动子的克隆研究打下了基础,目前国内外未见类似报道。

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