滴度

The four-week twice immunized hamsters acquired anti-MP in-fection capacity in local respiratory tract,as a result that swab culuturepositise was belove that of unimmunization group,and that only onehumster's lavage fluid culture was positive in immunization group.How-ever,unimmunization group positive rate of swabs and lavage fluid cul-ture was very high.This hinted that local respiratory tract's anti-MPinfection capacity was weak at the primary infection and the ability de-veloped gradually.4.The sIgA change in broncheal-lung lavage fluid.

而未免疫组咽拭子和支气管肺泡灌洗液MP分离培养的阳性均很高，说明在初次感染时，呼吸道局部防御清除MP的作用差，但随着时间的推移，其灌洗液培养的周次阳性率有下降趋势，MP培养的滴度亦随之降低，说明在感染以后随着时间的推移，呼吸道局部逐渐产生了防御、清除MP感染的作用。

The Antiviral substance was induced from blastulae embryonic cell line of crucian carp by ultra violet inactivated grass carp reovirus.

用紫外线灭活的草鱼呼肠孤病毒诱导鲫鱼囊胚细胞能产生一种高滴度的抗病毒物质。

In addition, the chimeric particular characteristics of the protsins and their immunogenicity were identified. It ...

用pCm－HCG免疫小鼠能产生高滴度的抗HCG抗体，说明核心抗原的中间部位是合适的融合位点。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

A double antigens Sandwich Enzymelinked Immunosor-bent Assay( DAgS -ELISA)have been developed under the basis o f higher titer and freeze dried production conjugate of Brucella antigen with horseradish peroxidase. Serumsamples formdiagnosed patients of Brucellosis and sheep,cattl e,swine infected with Bru-cella as well as normal men and those a nimals in laboratory detected throu gh DAgS -ELISA,SAT and RBPT tests.

方法在制备高滴度布氏菌抗原辣根过氧化物酶结合物及其冻干制品基础上，应用酶联免疫吸附双抗原夹心法即双抗原夹心酶联免疫吸附试验、试管凝集试验及虎红平板凝集试验对从山西省布氏菌病疫区收集的布氏菌病患者、布氏菌感染羊、牛、猪以及健康人、羊、牛、猪共计290份血清进行对比检测。

Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay. IgG avidity was measured by diethylamine dissociation ELISA.

血清IgG抗体滴度采用酶联免疫吸附试验测定；其亲和力通过二乙醇胺分离，ELISA测定。

Results Compared with the model group, GLGC can reduce the mortality of influenza virus pneumonia mice, prolong the average living days, inhibit the body weight decrease, and depress the lung index and viral titer distinguishingly.

结果 瓜蒌甘草颗粒治疗组较模型组死亡率明显降低，平均生存天数明显延长，体重下降得到明显改善，肺指数和血凝滴度都明显降低。

MethodsThe frequency of CD4+CD25+T cells in the peripheral blood mononuclear cells from 30 cases of severe hepatitis B, 20 cases of chronic hepatitis B, 20 cases of chronic asymptomatic HBV carriers and 10 healthy controls were determined by flow cytometry and their serum HBV DNA levels were detected by Quantitative Fluorogenic PCR.

方法采用流式细胞仪检测30例重型乙型肝炎患者、20例慢性乙型肝炎患者、20例无症状乙肝病毒携带者和10例健康的外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）的水平，并应用荧光定量PCR方法测定上述研究对象血中HBV DNA滴度。

Methods The glutin agglutination rection was used, the diluting power of antibody of Mycoplasma pneumoniae was more then 1:80, it was a positive sample.

目的 了解肺炎支原体在青少年中的感染情况方法采用明胶凝集的方法，若抗MP-IgM滴度≥1：80，阳性。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。