滋养细胞

On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明：早孕胎盘来源的细胞滋养层细胞在血清诱导下，首先发生聚集，并通过桥粒和细胞骨架使相邻的细胞膜紧密接触；之后细胞膜发生片段断裂，形成融合孔样结构；细胞间桥粒消失，最终形成多核的合体细胞；免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂（amitosis即细胞核分裂而细胞质不分裂）实现的。

This lump goes numb even if the body controls the growth of the trophoblastic cells and proves to be highly injurious if the cells keep spreading.

这一笔云麻木，即使身体控制生长的滋养层细胞，并证明，以高度的损害，如果细胞继续蔓延。

Results VEGF and PLGF were mainly expressed in trophoblast, next in decidua and endothelial cells, and also in stroma.

结果 各组孕妇胎盘组织中的VEGF和PLGF主要分布于滋养叶细胞，其次是蜕膜组织，绒毛血管内皮细胞和部分间质细胞也有表达。

BACKGROUND: The natural killer cells at the site of placentation express killer-cell immunoglobulin-like receptors that can bind to human leukocyte antigen-C molecules on trophoblast cells.

背景：自然杀伤细胞在胎盘形成时表达伤细胞免疫球蛋白样受体，它可以在滋养层细胞上与人白细胞抗原-C分子结合。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

The HBsAg was located in the intervillous space (21 placentas) and...

HBsAg主要分布于蜕膜血管内皮细胞（18例）、蜕膜细胞（21例）、滋养层细胞（8例）、绒毛间质细胞（8例）和绒毛血管内皮细胞（5例）的胞浆和绒毛间隙（21例）中。

It was expressed on trophoblastic cell, interstitial cell and endothelium cell of interstitial blood vessels of villi.

结果 ①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

To explore the existence and dynamic changes of the Golgi body-like structure in the log-phase and encysting trophozoites (6, 12, 18 hours) of Giardia lamblia, these trophozoites were stained with C6-NBD ceramide, a specific marker for Golgi body in eukaryotic cells, and then they were observed with fluorescence microscopy.

目的 探讨类高尔基体结构在蓝氏贾第鞭毛虫滋养体和成囊不同时段的存在情况及其动态变化机理。方法用可特异性标记真核细胞高尔基体的荧光染料C6-NBD神经酰胺，标记有活性的蓝氏贾第鞭毛虫滋养体及成囊6、12、18h虫体，并用荧光显微镜观察。

Lamblia were cultivated axenically with modified TYI-S-33 medium contained dihydroartemisinin (0.002 mg/L) for 12 h and then stained by rhodanmine phalloidin and paclitaxel (P22310). The cytoskeletons of the organisms were detected and analyzed by flow cytometry, and then observed by confocal microscopy.RESULTS: The cytoskeleton of trophozoites was markedly damaged after treatment with 0.002 mg/L dihydroartemisinin for 12 h.

将用含双氢青蒿素的改良TYI-S-33培养基培养后的蓝氏贾第鞭毛虫滋养体，用专一性结合f-肌动蛋白的荧光染料罗丹明－鬼笔环肽(rhodanmine phalloidin， RDP)标记微丝，结合微管的紫杉醇(Paclitaxel， Oregen Green 488， P22310)标记微管，用流式细胞术检测、分析，用激光共聚焦显微镜(confocal microscopy， CFM)观察滋养体微丝和微管的变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。