滋养核

On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明：早孕胎盘来源的细胞滋养层细胞在血清诱导下，首先发生聚集，并通过桥粒和细胞骨架使相邻的细胞膜紧密接触；之后细胞膜发生片段断裂，形成融合孔样结构；细胞间桥粒消失，最终形成多核的合体细胞；免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂（amitosis即细胞核分裂而细胞质不分裂）实现的。

The antiparasitic effect of caprylic acid against several fish parasites, i.e., the ciliate Cryptocaryon irritans, monogenean Benedenia seriolae, copepod Pseudocaligus fugu and myxosporean Kudoa shiomitsui, was examined by in vitro trials.Caprylic acid at a concentration of 1 mM had a parasiticidal effect against C. irritans theronts, B. seriolae oncomiracidia and K. shiomitsui spores and a contractile effect against B. seriolae adults, but had no clear effect against P. fugu copepodids and adults.

在体外实验中研究了辛酸对多种鱼类寄生虫的抗虫效果，如纤毛虫刺激隐核虫、单殖吸虫鰤贝尼登虫、桡足类Pseudocaligus fugu和微孢子虫库道虫。1mM的辛酸对刺激隐核虫滋养体、鰤贝尼登虫纤毛幼虫和库道虫孢子具有杀虫效果，对鰤贝尼登虫成虫有收缩效果，但对Pseudocaligus fugu的桡足幼体和成虫无明显效果。

The antiparasitic effect of caprylic acid against seeral fish parasites, i.e., the ciliate Cryptocaryon irritans, monogenean Benedenia seriolae, copepod Pseudocaligus fugu and myxosporean Kudoa shiomitsui, was examined by in itro trials.Caprylic acid at a concentration of 1 mM had a parasiticidal effect against C. irritans theronts, B. seriolae oncomiracidia and K. shiomitsui spores and a contractile effect against B. seriolae adults, but had no clear effect against P. fugu copepodids and adults.

在体外实验中研究了辛酸对多种鱼类寄生虫的抗虫效果，如纤毛虫刺激隐核虫、单殖吸虫鰤贝尼登虫、桡足类Pseudocaligus fugu和微孢子虫库道虫。1mM的辛酸对刺激隐核虫滋养体、鰤贝尼登虫纤毛幼虫和库道虫孢子具有杀虫效果，对鰤贝尼登虫成虫有收缩效果，但对Pseudocaligus fugu的桡足幼体和成虫无明显效果。

Objective: To investigate the relationship of PPARγ, MMP-2 and MMP-9 with trophoblast invasion.

目的：探讨Ⅱ型核受体的过氧化物酶体增殖物激活型受体γ、金属蛋白酶-2和MMP-9与细胞滋养细胞浸润的关系。

No significant correlation between nucleus roundness and trophozoite roundness.

滋养体圆度和核圆度之间无相关性。

An analysis revealed very significant positive correlation between trophozoite area and nucleus area (r=0.54, P .01), trophozoite volume and nucleus volume(r=0.47, P .01), and negative correlation between trophozoite area and trophozoite roundness (r=-0.29, P .01), no significant correlation between nucleus roundness and trophozoite roundness.

相关分析显示滋养体与核的面积(r=0 。5 4 ，P 。0 1)、滋养体与核体积(r=0 。4 7，P 。0 1)相关性有显著性意义，滋养体面积和核圆度呈负相关(r=- 0 。2 9，P 。0 1)，滋养体圆度和核圆度之间无相关性。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外，还可见到多种形态异常的虫体，包括；虫体呈非典型二分裂；滋养体胀大，胞质中有团块状和不规则形状的嗜酸性物质；在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛；在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛；在一个母体细胞中含有3或4个子体细胞的雏形；胞质互相融合的4个滋养体的雏形；胞质互相触合的3个滋养体，胞质互相融合的4个滋养体；1对营二分裂的滋养体与另一个滋养休互相融合；2对营二分裂的滋养体互相融合；仅有1个细胞核的滋养体。

Results Under TEM, the surface and nuclear membrane split, a great number of vacuoles and high electron density particles appeared in the cytoplasm in Pc trophozoites, vacuoles also did in Pc cysts, intracystic bodies became denaturation and necrosis; under SEM, filopodia and microvilli became sparse,short and fell off, a breach appeared in the surface membrane in Pc trophozoites.

结果 透射电镜发现Pc滋养体表膜和核膜破裂，胞质中出现大量空泡和高电子密度颗粒，Pc包囊中也出现空泡，囊内小体变性坏死；扫描电镜发现Pc滋养体丝状伪足和微绒毛稀疏、变短甚至脱落，表膜出现缺损。

It was found that only few log-phase trophozoites were labeled by means of C6-NBD-ceramide, however, most of the encysting trophozoites were labeled in perinuclear region.

结果 极少数蓝氏贾第鞭毛虫滋养体被C6-NBD神经酰胺标记；多数处于成囊6、12、18h时段的虫体均可见被特异标记成亮绿色的核周围区域。golgin245和Rab1在滋养体及成囊诱导6、12、24h的虫体中的表达水平无显著变化。

To explore the existence and dynamic changes of the Golgi body-like structure in the log-phase and encysting trophozoites (6, 12, 18 hours) of Giardia lamblia, these trophozoites were stained with C6-NBD ceramide, a specific marker for Golgi body in eukaryotic cells, and then they were observed with fluorescence microscopy.

目的 探讨类高尔基体结构在蓝氏贾第鞭毛虫滋养体和成囊不同时段的存在情况及其动态变化机理。方法用可特异性标记真核细胞高尔基体的荧光染料C6-NBD神经酰胺，标记有活性的蓝氏贾第鞭毛虫滋养体及成囊6、12、18h虫体，并用荧光显微镜观察。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失