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METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后,又通过DEAE离子交换层析纯化包涵体,包涵体在8 mol/L尿素变性溶解,在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中,通过简单的稀释复性,通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2,最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Dissolving the drug and excipients in a suitable solvent, generally water for injection.

把产品和赋形剂溶解于适当的溶媒中,通常是注射用水。

This is particularly important for fat-soluble vitamins.

这对脂肪溶解型的维生素特别重要。

Vitamins can be grouped according to the substance in which they are soluble: fat-soluble and water-soluble.

按维生素的溶解环境,维生素可以分为两大类:脂溶性和水溶性。

In the field of equipment manufacturers to provide users with three-way down film evaporator, centrifugal, pressure-spray drying tower, extraction, concentration, recovery, deployment, dissolved, storage, filter, transmission, CIP-cleaning and sanitation fluid valves, Clean pipeline accessories such as complete sets of equipment, is the design, manufacture, sale, installation works, technical advice and service in one enterprise.

在设备制造领域中为用户提供三效降膜式蒸发器、离心、压力式喷雾干燥塔、提取、浓缩、回收、调配、溶解、储存、过滤、输送、CIP在线清洗及卫生流体阀门、洁净管道配件等成套设备,是集设计、制造、销售、工程安装、技术咨询及售后服务于一体的企业。

The herbicides in the sample was extracted with ethyl acetate. The extract was decanted into a flask through a filter paper, then the filtered solution was concentrated to dry. The dry sample was dissolved and added to 5 mL with acetone-n-hexane (30:70, V/V). It was cleaned up by Florisil, concentrated again. The sample was dissolved and adjusted to 1mL with acetone.

匀浆后的草莓试样用乙酸乙酯进行提取,提取液经滤纸过滤后收集,再浓缩蒸干,用丙酮:正己烷(30:70, V/V)溶液溶解至5ml,经Florisil固相萃取柱净化,吹干后用丙酮定容,供气相色谱-质谱联用仪进行测定。

The relative composition measurement of bismuth telluridewas carried out in the method of flame atom_absorbed spectrum.

研究了在室温下用(1 +1 )HNO3溶解Bi—Te合金薄膜,用火焰原子吸收光谱法测定Bi,Te的含量。

That produced a greenhouse effect which, in turn, melted seabed structures known as methane clathrates, which trap that gas in ice.

温室效应产生,转而溶解了海底结构中的甲烷气水包合物(即甲烷气体被困在固态水中)。

That produced a greenhouse effect which, in turn, melted seabed structures known as methane clathrates, which trap that gas in ice.

温室效应产生后,转而溶解了称为甲烷气水包合物的海底构造体系。

On the basis of experiments, the mechanism of phosphorus gettering for crystalline sili...

这表明:磷吸杂主要是与过渡族金属的溶解、扩散和分凝有关。

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