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The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5, 50mM Tris-bufferru将包涵体溶解,再藉由稀释法使其复性,并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响,另外探讨添加剂是否对可溶蛋白回收率有所帮助。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解,分离提取包涵体,用8mol/L尿素溶解,纯化后进行梯度透析复性。

It is confirmed that realumination of SDY was caused by reinsertion of the extraframework aluminum into the zeolitic framework and some Si atoms in SDY framework can be removed in 1.0mol.dm^-^3 KOH solution.

证实了KOH溶液溶解非骨架铝后,铝原子重新进入SDY的骨架,导致Si/Al比降低。

This kind of oil wastewater is a complex system in which comprise solid impurity, liquid impurity and resolvable salt.

这种含油污水是一种含有固体杂质、液体杂质和溶解盐类等较为复杂的多相体系。

Basic physical-chemistry properties such as solubility ,surface activity, micelle thermodynamic property ,solubilization and the applied properties such as moisture adsorbability ,water retentivity, microemulsion , antibacterial activity , antitumor activity have been studied systematiclly.

研究了该类表面活性剂的基本物化性能如溶解性、表面活性、胶束热力学性质、增溶性能以及吸湿保湿性、微乳性能、抑菌抗菌性、抗肿瘤活性等应用性能。

The achieved results can provide a foundation for the further analysis of coupled stress-dissolving mechanism of rock salt.

研究成果为进一步研究岩盐的应力-溶解耦合机制奠定了试验依据以及理论基础。

The products are used for dispersing and stirring , substance for two or more liquid or power materials . It can also be used together with various type of sandhill .

产品说明:分散机系列本产品是对两种或多种液体和固体粉装物料进行搅拌、溶解和分散的专用设备,也可以与各种型号砂磨机配套使用。

Carbonate rock saprolite is formed in zones of alteration and made up of travertine and residual carbonate crystals.

碳酸盐岩腐岩形成于包气带,由原岩中的碳酸盐溶解后再次沉淀形成,主要由钙华和残余碳酸盐晶体组成。

If one cannot dissolve scar tissue, then one can only ascend so far as an adult.

如果你不能溶解伤疤,随后你只能提升到像成人提升那样的程度。

This stage is preceded by a mainly lytic phase and is followed by a "burnt out" sclerotic phase.

此期的前一期为溶解期,紧接着为硬化期。

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My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

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