溶血素

To characterize hematolysis of Enterococcus from sheep.

了解羊源肠球菌溶血素的特性。

Methods The mouse models bearing S180 sarcoma were treated with cadmium chloride with dosages of 0.25,1.00 and 4.00 mg.kg-1,the anti-tumor effect was evaluated by calculating of tumor weight;spleen and thymus indexes,carbon phage assay,lymphocyte transformed assay and hematolysis assay were used to measure the effect of cadmium chloride on immune function.

应用0.25、1.00及4.00 mg.kg-1的氯化镉处理S180肉瘤小鼠模型，检测瘤重确定氯化镉对S180肉瘤的抑瘤作用，采用胸腺指数、脾脏指数、碳粒廓清试验、淋巴细胞转化实验、半数溶血素实验检测小鼠免疫功能的变化。

In order to observe the effect of the chicken hydrolytic protein on immune function of mice, the NIH impuberal mice and mice were perfused stomachs with the samples separately at the cencentration of 0.28 and 0.56g/kg.d sustaining 7 or 14 days.

以健康NIH幼鼠和小鼠为模型，分别按0.28g0.56g／kg.d每天灌胃鸡水解蛋白一次，连续7d或14d后，观察鸡水解蛋白对胸腺指数、脾脏指数、巨噬细胞吞噬指数、吞噬百分率和溶血素生成水平等指标的影响。

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

Methods The experimental mouse was injected with high, middle, and low dosages of marasmius androsaceus po1ysaccharide 5, 10, 20 mg kg^(-1d^(-1) in the abdomen, and the comparison group was given the same volume of saline water i. p. once a day. After treatment for 14 consecutive days, the blood cell count instrument was used to measure the mice hemogram, the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed., the ability of the spleen lymphocytes proliferation was tested by MTT, and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice.

小鼠腹腔注射不同剂量的鬼毛针多糖5， 10， 20 mgkg^(-1d^(-1)，对照组给予等量生理盐水，每天给药1次，连续14d后，用血细胞计数仪检测小鼠血象；用腹腔巨噬细胞吞噬鸡红细胞实验、碳粒廓清实验观察小鼠吞噬细胞的吞噬能力；采用MTT法测定小鼠脾T淋巴细胞增殖能力；采用血清溶血素形成实验观察鬼毛针多糖对小鼠特异性体液免疫功能的影响。

Methods Anti-DNase B antibodies of 87 patients with acute rheumatic fever and 202 rheumatic heart disease patients with rheumatic activity were detected with anti-DNase B antibody microtitration test,and compared with antistreptolysin "O" test.

采用抗DNA酶B微量法和自制试剂测定急性风湿热87例、活动期风湿性心脏病202例病人血清的抗DNA酶B抗体，与抗溶血素O抗体对照研究。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算，连续10d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算，连续10 d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

MethodsTo observe the effects of Xiaoshui powder on promoting extinction of tuberculous pleural fluid of Mus musculus albus with the administration route of lavage,counteracting Chronic hyperplastic inflammation ,and in their weight of thymus gland and spleen of mouse, as well as the level of serumal hemolysin.

方法运用灌胃给药途径观察其对小鼠在促进结核性胸水的消退、抗慢性增生性炎症中的作用及其对小鼠胸腺、脾脏的重量和血清溶血素水平的影响。

Rays,the humoral cellular and non-specific immunity in mice were all decreased. Genistein could facilitate recover all the above indices in the following aspect: lymphocyte stimulation index, delated hypersensitivity, production of antibodies, colony forming units- spleen, and spleen index.

4Gen可明显增加辐射小鼠脾淋巴细胞增殖指数、二硝基氟苯诱发耳廓肿胀率、血清溶血素水平、脾指数，使碳粒廓清指数、胸腺指数比对照组有增加的趋势。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。