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溶血素

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For normal mouse, Yi Shen Granule could prolong the swimming time of mouse, the survival in Yi Shen Granule groups was more than that in model control group in same cold condition. For low-immune mouse, The eliminated rate of carbon granule in Yi Shen Granule groups rats was faster than that in model control group rats, it showed that phagocytosis was boosted up, the absorbency of upside liquid in Yi Shen Granule groups rats was more than that in model control group rats in hemolysin mensurated test, it indicated that more antibody was come into being by giving Yi Shen Granule.

益肾颗粒可以延长小鼠游泳时间;增加在同一寒冷条件下,相同时间内小鼠的存活数;对免疫力低下小鼠,给药组动物的碳粒廓清指数增大,反映吞噬功能增强;在血清溶血素含量测定试验中,给药组动物的上清液吸收值增大,说明机体产生的抗体较多。

Methods: To make the model of deficiency of kidney-Yin Jimpy mice with Hydrocortisone and observe the influence on T-lymphocyte dyeing rate, englobement carbon particle, haemolysin production and delayed type hypersensitivity.

用氢化可的松制作小鼠肾阴虚模型,观察补肾活血方对T淋巴细胞染色率、吞噬碳粒、溶血素生成和迟发型超敏反应的影响。

RESULTS: High and medium dose (30, 10 μg/kg) of selenocarrageenan syrupus increased mononuclear phagocyte englobement function, T lymphocyte proliferating function and delayed hypersensitivity significantly. All 3 doses increased serum haemolysin, IL-1, 2 activity and B lymphocyte proliferating function significantly.

结果:硒化卡拉胶口服液(30、10μg/kg)均可增强T淋巴细胞增殖能力及DTH;硒化卡拉胶口服液30、10、5μg/kg均可升高单核巨噬细胞吞噬功能、血清半数溶血素值,增强B淋巴细胞增殖能力及IL-1、IL-2活性。

We tested the mononuclear phagocyte englobement function, serum haemolysin, delayed hypersensitivity, interlukine-1, 2(IL-1, 2) activity and T, B lymphocyte proliferating function.

测定小鼠单核巨噬细胞吞噬功能、血清半数溶血素值、迟发型变态反应、T淋巴细胞及B淋巴细胞增殖能力、白介素-1(IL-1)及白介素-2(IL-2)活性。

Results α-Linolenic acid was capable of increasing the quantity of antibody-producing cells and the level of serum hemolysin, promoting the expurgation effect and the phagocytic function of monocyte/macrophage, stimulating...

结果α-亚麻酸能提高小鼠的抗体生成细胞数和血清溶血素水平,促进小鼠的单核-巨噬细胞的碳廓清和腹腔巨噬细胞的吞噬能力,促进小鼠的迟发型变态反应,提高小鼠NK细胞的活性。结论α-亚麻酸具有增强小鼠免疫力作用。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the activity

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Results Sheep spleen polypeptides could significantly stimulate multiplication and transformation of mice's spleen lymphocytes; increased markedly the weight of spleen and the thymus; promoted delayed hypersensitivity; increased the quantity of antibody-producing cells; increased the level of serum hemolysin; promoted the expurgation effect and the phagocytic function of monocyte/macrophage and increased the activity of natural killer.

结果 羊脾多肽能使小鼠脾、胸腺增重,明显刺激小鼠的脾淋巴细胞增值,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,但促进小鼠单核-巨噬细胞碳廓清作用不明显,增强小鼠的单核-腹腔巨噬细胞吞噬能力,有效提高小鼠自然杀伤细胞活性。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the acˉtivity of natural killer.

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

With rabbit antiserum against ECP of reference strain JEL4,ECPs of 25 Et strains were detected by Dot-ELISA; additionally,alI strains had been detected both on their virulent factor Excellular Products, including the hemolysin and extracellular protease, and on their pathogenicity to mice and Xiphophorus helleri.

以Et强致病株JEL4的胞外产物ECP为抗原制备兔抗血清,用于Dot-ELISA检测25株Et的ECP,同时对Et的溶血素和胞外蛋白酶进行检测,结合小鼠和剑尾鱼的致病性试验。

The recombinant hemolysin can be induced to express at 17, 25 and 37℃, and the times to obtain the highest quantities of protein were 9, 6 and 3 h, respectively.

在17,25和37℃时重组的VHH溶血素蛋白都能得到很好的表达,达到最大表达量的时间分别为9,6和3h。

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